长链非编码RNA小核仁RNA宿主基因18在乙型肝炎病毒相关性肝癌病人血清中的表达及其预后意义  

Expression and prognostic significance of lncRNA SNHG18 in serum of patients with hepatitis B virus associated liver cancer

作　　者：李轶 尚立 LI Yi;SHANG Li(Department of Vascular Intervention,The Ninth Hospital of Xi'an,Xi'an,Shaanxi 710054,China;Department of Gastroenterology,The Ninth Hospital of Xi'an,Xi'an,Shaanxi 710054,China)

机构地区：[1]西安市第九医院血管介入科,陕西西安710054 [2]西安市第九医院消化内科,陕西西安710054

出　　处：《安徽医药》2024年第8期1586-1590,共5页Anhui Medical and Pharmaceutical Journal

基　　金：西安市第九医院科研项目(2020-7)。

摘　　要：目的探讨长链非编码RNA小核仁RNA宿主基因18(lncRNA SNHG18)在乙型肝炎病毒相关性肝癌(HBV相关HCC)病人血清中表达变化及与预后的相关性。方法选取2015年1月至2016年10月西安市第九医院乙型肝炎病人192例,分为乙型肝炎肝硬化106例(肝硬化组),乙型肝炎肝癌86例(肝癌组),同时选取正常对照组110例。采用实时荧光定量逆转录聚合酶链反应(qRT-PCR)法检测血清中lncRNA SNHG18相对表达水平;采用Pearson相关性分析法分析HBV相关肝癌病人血清lncRNA SNHG18与甲胎蛋白(AFP)、谷丙转氨酶(ALT)的相关性;采用Kaplan-Meier法分析HBV相关肝癌血清lncRNA SN⁃HG18表达与病人预后的关系;多因素Cox回归分析法分析HBV相关肝癌病人预后的影响因素。结果对照组、肝硬化组、肝癌组血清lncRNA SNHG18水平(1.00±0.21、0.75±0.18、0.46±0.09)依次显著降低(P<0.05);TNM分期为Ⅲ+Ⅳ期、发生淋巴结转移、浸润深层的HBV相关肝癌病人lncRNA SNHG18低表达的比例高于TNM分期为Ⅰ+Ⅱ期、未发生淋巴结转移、浸润浅层者(P<0.05)。Pearson相关分析显示,HBV相关肝癌病人血清lncRNA SNHG18水平与AFP、ALT均呈负相关(r=−0.41、−0.54,P<0.001);lncRNA SNHG18高表达HBV相关肝癌病人5年生存率高于lncRNA SNHG18低表达病人(P<0.001);lncRNA SNHG18[HR=0.86,95%CI:(0.78,0.96)]、TNM分期[HR=2.76,95%CI:(1.13,6.74)]是HBV相关肝癌病人预后不良的独立影响因素(P<0.05)。结论HBV相关肝癌病人血清lncRNA SNHG18低表达,且lncRNA SNHG18低表达是预后不良的危险因素。Objective To investigate the expression changes of long non-coding RNA small nucleolar RNA host gene 18(lncRNA SNHG18)in serum of patients with hepatitis B virus-related liver cancer(HBV-related HCC)and its correlation with prognosis.Methods One hundred and ninety-two patients with hepatitis B in the Ninth Hospital of Xi'an from January 2015 to October 2016 were selected,and they were assigned into 106 cases of hepatitis B cirrhosis,(liver cirrhosis group)and 86 cases of hepatitis B liver cancer(HCC group),meantime,110 normal control groups were selected.The relative expression level of lncRNA SNHG18 in serum was detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction(qRT-PCR)method.The correlation between serum lncRNA SNHG18 and alpha-fetoprotein(AFP)and alanine aminotransferase(ALT)in HBV-associated HCC patients was analyzed by Pearson correlation analysis.Kaplan-Meier method was used to analyze the relationship between the expression of serum lncRNA SNHG18 in HBV-related HCC and the prognosis of patients.Multivariate Cox regression analysis was used to analyze the prognostic factors of HBV-related HCC patients.Results The level of serum lncRNA SNHG18 in the control group,liver cirrhosis group and HCC group was greatly decreased in turn(1.00±0.21,0.75±0.18,0.46±0.09,P<0.05);the proportion of low expression of lncRNA SNHG18 of HBV-related HCC patients with TNM stageⅢ+Ⅳ,lymph node metastasis,and deep invasion was greatly higher than that of patients with TNM stageⅠ+Ⅱ,no lymph node metastasis,and superficial invasion(P<0.05).Pearson correlation analysis showed that serum lncRNA SNHG18 level in HBV-related HCC patients was negatively correlated with AFP and ALT(r=−0.41,−0.54,P<0.001);the 5-year survival rate of patients with HBV-related HCC with high expression of lncRNA SNHG18 was higher than that of patients with low expression of lncRNA SNHG18(P<0.001);lncRNA SNHG18[HR=0.86,95%CI:(0.78,0.96)]and TNM stage[HR=2.76,95%CI:(1.13,6.74)]were independent influencin

关 键 词：肝肿瘤 乙型肝炎病毒 核糖核酸 小核仁 甲胎蛋白 谷丙转氨酶 危险因素 预后 

分 类 号：R735.7[医药卫生—肿瘤] R512.62[医药卫生—临床医学]