Surfactant-assisted crystallization of lysozyme and its mechanism  

作　　者：Yujie Song Rongfei Ren Min Su Zhihan Liang Qi Hao 

机构地区：[1]School of Chemical Engineering,Hebei University of Technology,Tianjin 300130,China

出　　处：《Particuology》2024年第7期199-208,共10页颗粒学报（英文版）

基　　金：National Natural Science Foundation of China(grant No.21878067).

摘　　要：Surfactants were used to assist in the crystallization of lysozyme.Three types of surfactants with different structures(sodium dodecyl sulfate,cetyltrimethylammonium bromide,or betaine hydrochloride[BH])were used.The study indicated that three surfactants were all capable of shortening the crystallization induction time of lysozyme,with BH inducing the shortest crystallization induction time(t=1 min)and the largest crystal size(37μm).The molecular interactions among each of the three surfactants aforementioned and lysozyme were also investigated by simulations.It was found that hydrogen bonding dominantly acted between the carboxyl structure of the surfactant BH and the lysozyme,which produced the most favorable conformation for the self-assembly of lysozyme(promote lysozyme orderly aggregation and shorten the crystallization induction time).On this basis,the mechanism of surfactant-promoted lysozyme crystallization was proposed.

关 键 词：PROTEIN CRYSTALLIZATION SURFACTANT LYSOZYME Molecular interaction MECHANISM 

分 类 号：O64[理学—物理化学]