Development of a stable attenuated double-mutant of tobacco mosaic virus for cross-protection  

作　　者：Xiaojie Xu Shaoyan Jiang Chunju Liu Xujie Sun Qing Zhu Xiuzhai Chen Pengchao Jiang Fenglong Wang Yanping Tian Xiangdong Li 

机构地区：[1]The Engineering Research Institute of Agriculture and Forestry,Ludong University,Yantai 264025,China [2]Laboratory of Plant Virology,Department of Plant Pathology,College of Plant Protection,Shandong Agricultural University,Tai’an 271018,China [3]Weifang Tobacco Corporation,Weifang 261031,China [4]Linyi Tobacco Corporation,Linyi 276000,China [5]Tobacco Research Institute,Chinese Academy of Agricultural Sciences,Qingdao 266101,China

出　　处：《Journal of Integrative Agriculture》2024年第7期2318-2331,共14页农业科学学报（英文版）

基　　金：supported by funds from‘Taishan Scholar’Construction Project,China(TS2022-028 and 202101KN275)。

摘　　要：Tobacco(Nicotiana tabacum)and tomato(Solanum lycopersicum)are two major economic crops in China.Tobacco mosaic virus(TMV;genus Tobamovirus)is the most prevalent virus infecting both crops.Currently,some widely cultivated tobacco and tomato cultivars are susceptible to TMV and there is no effective strategy to control this virus.Cross-protection can be a safe and environmentally friendly strategy to prevent viral diseases.However,stable attenuated TMV mutants are scarce.In this study,we found that the substitutions in the replicase p126,arginine at position 196(R^(196))with aspartic acid(D),glutamic acid at position 614(E^(614))with glycine(G),serine at position 643(S^(643))with phenylalanine(F),or D at position 730(D^(730))with S,significantly reduced the virulence and replication of TMV.However,only the mutation of S^(643) to F reduced the RNA silencing suppression activity of TMV p126.A double-mutant TMV-E614G-S643F induced no visible symptom and was genetically stable through six successive passages in tobacco plants.Furthermore,our results showed that TMV-E614G-S643F double-mutant could provide effective protection against the wild-type TMV infection in tobacco and tomato plants.This study reports a promising mild mutant for cross-protection to control TMV in tobacco and tomato plants.

关 键 词：CROSS-PROTECTION double-mutant p126 tobacco mosaic virus VIRULENCE 

分 类 号：S432.41[农业科学—植物病理学]