Dynamic transcriptome profiles and novel markers in bovine spermatogenesis revealed by single-cell sequencing  

作　　者：Yuan Gao Fuxia Bai Qi Zhang Xiaoya An Zhaofei Wang Chuzhao Lei Ruihua Dang 

机构地区：[1]Key Laboratory of Animal Genetics,Breeding and Reproduction of Shaanxi Province,College of Animal Science and Technology,Northwest A&F University,Yangling 712100,China [2]College of Life Sciences,Northwestern Polytechnical University,Xi’an 710072,China

出　　处：《Journal of Integrative Agriculture》2024年第7期2362-2378,共17页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China(81770514);the 2020 Scientist Plus Engineer Program of Shaanxi Province,China(K4050422468);the Key Research&Development Plan of Shaanxi Province,China(2020NY-002);the Natural Science Basis Research Plan of Shaanxi Province,China(2023-JC-QN-0242);the China Agriculture Research System of MOF and MARA(CARS-37)。

摘　　要：Testicular development is an important biological process in male and requires interaction between the male germ cells and somatic cells.However,the mechanisms of testicular development in livestock,particularly in cattle,are poorly understood.Furthermore,cellular heterogeneity hinders the profiling of different cell types at different developmental stages.In this study,we first performed a single-cell transcriptomic study of the bovine testis development during puberty by using 10×genomics single-cell RNA sequencing(scRNA-seq).By collecting the scRNA-seq data from 11,083 cells from prepubertal and pubertal bovine testes,a high-resolution scRNA-seq atlas was described,identifying 9 somatic and 13 spermatogenic clusters.We also distinguished several stage-specific marker genes for bovine germ cells and somatic cells,such as GRAF2 and MORC1 for SSC(spermatogonial stem cells),HJURP and TCF19 for differentiating spermatogonia,ARSE for immature Sertoli,CLEC12B for mature Sertoli,LOC112441470 for Leydig.In conclusion,we have examined the transcription levels and constructed the single-cell developmental maps of germ cells and somatic cells during testicular development in Angus cattle.The datasets provided new insights into spermatogenesis and testicular somatic cell development in cattle.

关 键 词：scRNA-seq CATTLE SPERMATOGENESIS testis development 

分 类 号：S823.2[农业科学—畜牧学]