Lnc RNA SNHG14通过调节miR-206表达来促进卵巢癌的发展  

Lnc RNA SNHG14 promotes the development of ovarian cancer by regulating miR-206 expression

作　　者：崔静 贾志燕 王立君 郭姝媛 CUIJing;JIA Zhi-yan;WANG Li-jun(Operating Room,Jiyang District People's Hospital,Jinan 251400,China)

机构地区：[1]山东省济南市济阳区人民医院手术室,251400 [2]山东省济南市济阳区人民医院妇产科,251400 [3]山东省济南市济阳区人民医院彩超室,251400

出　　处：《中国现代药物应用》2024年第13期170-174,共5页Chinese Journal of Modern Drug Application

基　　金：济南市卫生健康委员会第二批科技计划项目(项目编号:2020-4-163)。

摘　　要：目的探讨长链非编码RNA(Lnc RNA)小核仁RNA宿主基因14(SNHG14)在卵巢癌组织中的表达,以及Lnc RNA SNHG14通过微小RNA(miR)-206影响卵巢癌的增殖、迁移和侵袭能力。方法收集58例行卵巢癌根治手术患者的癌组织及其邻近的癌旁组织(距离癌组织>3 cm),将其进行细胞培养,待细胞融合度达到70%左右时进行细胞转染,分别转染阴性对照质粒(si-NC组)、SNHG14高表达质粒(si-SNHG14组);采用实时荧光定量聚合酶链式反应(qRT-PCR)检测卵巢癌组织与癌旁组织中SNHG14、miR-206表达量,采用细胞增殖活性检测试剂盒(CCK-8)实验、平板克隆形成实验、Matrigel侵袭实验分别检测SNHG14对卵巢癌细胞的增殖能力、克隆形成数、迁移及侵袭;双荧光素酶报告实验检测Lnc RNA SNHG14与miR-206的靶向关系。结果与癌旁组织的(1.01±0.08)、(1.00±0.07)比较,卵巢癌组织中SNHG14表达量(2.56±0.57)升高、而miR-206表达量(0.36±0.11)降低(P<0.05);通过Pearson法检测卵巢癌组织中SNHG14与miR-206的相关性显示,SNHG14与miR-206呈负相关(r=-0.803,P<0.01)。与si-NC组的(1.01±0.06)、(1.00±0.08)比较,si-SNHG14组的SNHG14表达量(0.27±0.07)降低、miR-206表达量(2.96±0.45)升高(P<0.05);si-SNHG14组的吸光度(OD值)(0.29±0.05)较si-NC组的(0.79±0.06)降低,克隆形成数(52.35±7.37)较si-NC组的(120.51±19.34)减少(P<0.05)。si-SNHG14组的迁移细胞数(43.11±5.87)、侵袭细胞数(49.66±11.01)均较si-NC组的(117.11±10.48)、(122.30±12.97)明显减少(P<0.05)。采用starbase预测与SNHG14可互补结合的miR-206。转染miR-206 mimics可降低含有野生型载体的卵巢癌细胞的荧光素酶活性(P<0.05),而未能抑制含有突变型载体的卵巢癌细胞的荧光素酶活性(P>0.05)。结论卵巢癌组织和卵巢癌细胞中Lnc RNA SNHG14的表达会升高,Lnc RNA SNHG14可靶向miR-206,从而调节卵巢癌细胞的增殖、克隆形成、迁移和侵袭。Objective To explore the expression of long chain non-coding RNA(Lnc RNA)small nuclear RNA host gene 14(SNHG14)in ovarian cancer tissue and the impact of Lnc RNA SNHG14 on the proliferation,migration,and invasion ability of ovarian cancer through micro RNA(miR)-206.Methods The cancer tissues and their adjacent paracancerous tissues(>3 cm away from the cancer tissues)of 58 cases of patients undergoing radical surgery for ovarian cancer were collected for cell culture,and transfected with negative control plasmid(si-NC group)and SNHG14 high expression plasmid(si-SNHG14 group)when the degree of cell fusion reached about 70%.Quantitative real-time fluorescent polymerase chain reaction(qRT-PCR)was used to detect the expression levels of SNHG14 and miR-206 in ovarian cancer tissues and their adjacent paracancerous tissues;the cell counting kit-8(CCK-8)assay,plate clone formation assay,and Matrigel invasion assay were used to detect the proliferation,clone formation,migration,and invasion of SNHG14 on ovarian cancer cells;the dual luciferase assay was used to detect the targeting relationship between Lnc RNA SNHG14 and miR-206.Results Compared with(1.01±0.08)and(1.00±0.07)in paracancerous tissues,SNHG14 expression of(2.56±0.57)was elevated and miR-206 expression of(0.36±0.11)was decreased in ovarian cancer tissues(P<0.05).The correlation between SNHG14 and miR-206 in ovarian cancer tissues detected by Pearson's method showed that SNHG14 was negatively correlated with miR-206(r=-0.803,P<0.01).Compared with(1.01±0.06)and(1.00±0.08)in the si-NC group,the expression level of SNHG14[(0.27±0.07)]was decreased and the expression level of miR-206[(2.96±0.45)]was increased in the si-SNHG14 group(P<0.05).The si-SNHG14 group had lower optical density(OD)of(0.29±0.05)than(0.79±0.06)in si-NC group,and lower clone formation of(52.35±7.37)than(120.51±19.34)in si-NC group(P<0.05).The number of migrating cells and invading cells were(43.11±5.87)and(49.66±11.01)in si-SNHG14 group,which were significantly lower than(117.11±

关 键 词：长链非编码RNA 小核仁RNA宿主基因14 微小RNA-206 卵巢癌细胞 增殖 克隆形成 迁移 侵袭 

分 类 号：R737.31[医药卫生—肿瘤]