2种抗生素对大黄鱼肠道组织及紧密连接蛋白基因表达的影响  

Effects of Two Antibiotics on Intestinal Histological Structure and Tight Junction Protein Gene Expression in Large Yellow Croaker Larimichthys crocea

作　　者：成好学 王庚申[2] 许叶祥 王莹 刘慧玲 徐伟 施慧[2] 谢建军[2] 汪玮[2] 张东旭 许文军[2] CHENG Haoxue;WANG Gengshen;XU Yexiang;WANG Ying;LIU Huiling;XU Wei;SHI Hui;XIE Jianjun;WANG Wei;ZHANG Dongxu;XU Wenjun(School of Fishery,Zhejiang Ocean University,Zhoushan 316022,China;Zhejiang Province Key Laboratory of Mariculture and Enhancement,Zhejiang Marine Fisheries Research Institute,Zhoushan 316021,China)

机构地区：[1]浙江海洋大学水产学院,浙江舟山316022 [2]浙江省海洋水产研究所,浙江省海水增养殖重点实验室,浙江舟山316021

出　　处：《水产科学》2024年第4期531-540,共10页Fisheries Science

基　　金：国家重点研发计划项目(2020YFD0900803);浙江省三农九方科技协作项目(2022SNJF70);浙江省基础公益研究计划项目(LGN21C190006);浙江省农业农村厅协同创新项目(2020XTTGSC04)。

摘　　要：为探究盐酸多西环素和恩诺沙星对大黄鱼肠道结构及肠道紧密连接蛋白相关基因表达的影响,在水温16.80~22.50℃下,将体质量(41.57±1.63)g的大黄鱼饲养在直径1 m、水深1.25 m的圆形塑料水槽中,投喂剂量0、0.2、1.0、2.0 g/kg的药饵5~7 d,停药后1、5、15 d,观察肠道组织形态变化,测定紧密连接蛋白基因Claudin-7、ZO-1和Occludin的相对表达量。试验结果显示:大黄鱼摄食2种药物后,肠道均出现肠绒毛萎缩,组织空泡化等病变,随着给药剂量的增加和给药时间的延长,肠道组织损伤程度逐渐加深,紧密连接蛋白基因表达量持续降低;肠黏膜损伤在停药15 d后仍未恢复至对照组水平,药物剂量越高恢复程度越低;与对照组相比,2种药物高剂量组紧密连接蛋白基因表达量在停药15 d后仍显著低于对照组(P<0.05),其余组无显著差异(P>0.05)。试验结果表明,盐酸多西环素和恩诺沙星以剂量时间依赖方式损伤大黄鱼肠道组织,降低肠道通透性,药物剂量越高,恢复周期越长,在15 d后仍未完全恢复。In order to explore the effects of doxycycline hydrochloride and enrofloxacin on the intestinal histological structure and expression of intestinal tight junction proteins genes in large yellow croaker Larimichthys crocea,the large yellow croaker with body weight of(41.57±1.63) g was reared in a circular plastic tank with a diameter of 1 m and water depth of 1.25 m,and fed the diets containing both drugs at a dose of 0,0.2,1.0 and 2.0 g/kg at water temperature of 16.80—22.50 ℃ for 5—7 days.The morphological changes in intestinal histology were observed and the relative expression levels of tight junction protein genes Claudin-7,ZO-1 and Occludin were detected in the large yellow croaker fed the medicated diets 1,5 and 15 days after the drug was stopped to be fed.The results showed that the large yellow croaker fed the medicated diets had intestinal villus atrophy and tissue vacuolization,with gradually elevated in the degree of intestinal tissue damage with the increase in dose and prolongation of the administration time.The expression levels of the tight junction protein gene were shown to be decreased.The intestinal mucosal injury was not found to be recovered to the level of the control group 15 days after drug withdrawal,with the higher at the higher drug concentration.There was still significantly lower expression level of tight junction protein gene in the high-dose group than that in the control group at 15 days after withdrawal(P<0.05).In conclusion,doxycycline hydrochloride and enrofloxacin caused intestinal tissue damage and reduced intestinal permeability in large yellow croaker in a dose-time dependent manner,and the higher intestinal and the damage still did not fully recover 15 days after administration.

关 键 词：盐酸多西环素 恩诺沙星 大黄鱼 肠道 组织病理 紧密连接蛋白基因 

分 类 号：S942.26[农业科学—水产养殖]