人参果汁浓缩液对环磷酰胺诱导免疫抑制小鼠免疫功能的影响  

Effects of Panax ginseng fructus juice concentrate on immune injury induced by cyclophosphamide in mice

作　　者：石峻岭 孙宇立[1] 郑志清 王婷 苏怡 SHI Junling;SUN Yuli;ZHENG Zhiqing;WANG Ting;SU Yi(Shanghai Institue of Preventive Medicine,Shanghai 200336,China)

机构地区：[1]上海市预防医学研究院,上海200336

出　　处：《中国食品卫生杂志》2024年第4期377-382,共6页Chinese Journal of Food Hygiene

摘　　要：目的研究人参果汁浓缩液对环磷酰胺致小鼠免疫损伤的作用及其可能机制。方法将72只SPF级雄性C57BL/6J小鼠随机分成空白对照组、环磷酰胺组、阳性药物组、人参果汁浓缩液低、中、高剂量组(83、166、249 mg/kg),每组12只。第1~14天,人参果汁浓缩液3个剂量组小鼠灌胃相应的人参果汁浓缩液,空白对照组和环磷酰胺组小鼠灌胃等量的水;第1~7天,阳性药物组小鼠灌胃等量的水,第8~14天,阳性药物组小鼠灌胃20 mg/kg盐酸左旋咪唑(LH);第12~14天,环磷酰胺组、阳性药物组和人参果汁浓缩液各剂量组小鼠腹腔注射80 mg/kg环磷酰胺,空白对照组腹腔注射等量的生理盐水。实验结束后,计算小鼠脾脏指数、测定小鼠外周血白细胞(WBC)、淋巴细胞(LYM)和LYM%以及检测小鼠血清和脾脏中乳酸脱氢酶(LDH)和酸性磷酸酶(ACP)活性、小鼠脾脏中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性和丙二醛(MDA)含量。结果各组小鼠体质量比较,差异无统计学意义(P>0.05)。与空白对照组比较,环磷酰胺组小鼠脾脏指数降低(P<0.01)、外周血WBC、LYM和LYM%降低(P<0.05)、血清和脾脏中ACP活性降低(P<0.05)或呈降低趋势、脾脏中SOD、CAT活性降低、MDA含量升高(P<0.05);与环磷酰胺组比较,人参果汁浓缩液各剂量组脾脏指数升高(P<0.05,P<0.01)、人参果汁浓缩液低、高剂量组LYM%升高(P<0.05)、人参果汁浓缩液各剂量组血清和脾脏组织中ACP活性呈升高趋势或升高(P<0.05)、人参果汁浓缩液低剂量组SOD和CAT活性呈升高趋势、人参果汁浓缩液各剂量组MDA含量降低(P<0.05,P<0.01)。结论人参果汁浓缩液在一定程度上可改善环磷酰胺引起的小鼠免疫损伤作用,可能与减轻小鼠免疫器官氧化损伤作用相关。Objective To study the effect and possible mechanism of Panax ginseng fructus juice concentrate on immune injury induced by cyclophosphamide in mice.Methods Seventy-two specific pathogen-free(SPF)C57BL/6J male mice were randomly divided into six groups(12 per group),including blank group,cyclophosphamide group,positive drug group,low-,middle-and high-dose Panax ginseng fructus juice concentrate groups(83,166,249 mg/kg).From day 1 to day 14,mice in three groups of Panax ginseng fructus juice concentrate were intragastrically administrated with concentrate at volumes of corresponding dosage,and mice in blank group and cyclophosphamide group were gavaged with the same amount of distilled water.Mice in positive drug group were also intragastrically administrated with the same amount of water from day 1 to day 7,and then from day 8 to day 14,gavaged with 20 mg/kg levamisole hydrochloride(LH).From day 12 to day 14,mice in cyclophosphamide group,positive drug group and three Panax ginseng fructus juice concentrate groups were intraperitoneally injected with 80 mg/kg cyclophosphamide,while those in blank group were injected with the same amount of normal saline.At the end of the experiment,the spleen index was calculated,leukocyte(WBC),lymphocyte(LYM)and LYM%in peripheral blood were measured,and the activities of lactate dehydrogenase(LDH)and acid phosphatase(ACP)in serum and spleen,activities of superoxide dismutase(SOD),catalase(CAT)and malondialdehyde(MDA)content in spleen homogenate were measured.Results There was no significant difference in body weight among groups(P>0.05).Compared with blank group,the spleen index of mice decreased(P<0.01),WBC,LYM and LYM%decreased,the activity of ACP in serum and spleen of mice decreased or showed a downward trend,the activity of SOD and CAT in spleen decreased and the content of MDA increased in cyclophosphamide group(P<0.05).Compared with cyclophosphamide group,spleen index of mice in the panax ginseng fructus juice concentrate groups increased(P<0.05,P<0.01),LYM%in the low-and

关 键 词：人参果 环磷酰胺 免疫抑制 氧化损伤 免疫调节 

分 类 号：R155[医药卫生—营养与食品卫生学]