实时荧光PCR检测弯曲菌感染病例的粪便和肛拭子样本  

Detection of the stool and anal swab samples from outbreaks caused by Campylobacter by real-time PCR

作　　者：邹林[1] 贾楠 张萍[1] 甄博珺[1] 郭晓晨 王芳[1] 冀国强[2] 闫爱霞 康颖 马红梅[2] 李颖[2] ZOU Lin;JIA Nan;ZHANG Ping;ZHEN Bojun;GUO Xiaochen;WANG Fang;JI Guoqiang;YAN Aixia;KANG Ying;MA Hongmei;LI Ying(Tongzhou District Center for Disease Prevention and Control,Beijing 101100,China;Shunyi District Center for Disease Prevention and Control,Beijing 101300,China)

机构地区：[1]北京市通州区疾病预防控制中心,北京101100 [2]北京市顺义区疾病预防控制中心,北京101300

出　　处：《中国食品卫生杂志》2024年第4期389-393,共5页Chinese Journal of Food Hygiene

摘　　要：目的通过对3起弯曲菌暴发事件中粪便和肛拭子样本采用实时荧光PCR和培养法检测结果的比较,为实验室快速有效地应对此类事件奠定基础。方法收集3起弯曲菌感染暴发事件中病例的生物样本;使用过滤培养法进行弯曲菌培养检测,分别使用原始样本、增菌24 h和增菌48 h样本提取DNA进行弯曲菌实时荧光PCR检测;使用Kappa检验对实时荧光PCR检测结果和培养法结果进行一致性分析。结果原始样本、增菌24 h和增菌48 h实时荧光PCR检测灵敏度分别为90.91%、97.22%和100%,特异度分别为75.00%、84.00%和78.95%,与培养法结果一致性分析的Kappa值分别为0.643、0.813和0.785。结论实时荧光PCR检测与培养法结合使用是弯曲菌暴发事件处置的有效实验室检测方法。Objective To lay the foundation of quickly and effectively response to such epidemics for laboratories,the detection of the real-time PCR and culture test results of stool and anal swab samples from 3 outbreaks caused by Campylobacter was compared.Methods Biological samples from the 3 outbreaks of Campylobacter were collected.Bacterial culture was performed on the samples using filter culture method.DNA was extracted from the original samples,24 h enriched samples and 48 h enriched samples.Kappa test was used to analyze the results of real-time PCR and culture method.Results The real-time PCR detection sensitivity of original samples,24 h enriched samples and 48 h enriched samples were 90.91%,97.22%and 100%.And the detection specificity were 75.00%,84.00%和78.95%,respectively.Kappa value for the consistency analysis in culture method with real-time PCR based on original samples,24 h enriched culture samples and 48 h enriched samples were 0.643,0.813 and 0.785,respectively.Conclusion The combination of real-time PCR detection and culture detection is an effective laboratory testing method for the respond of Campylobacter outbreaks.

关 键 词：弯曲菌 荧光定量聚合酶链式反应 培养法 腹泻病例 食源性致病菌 

分 类 号：R155[医药卫生—营养与食品卫生学]