马铃薯StNF-YA9基因的生物信息学及其表达分析  

Bioinformatics and Expression Analysis of the StNF-YA9 Gene in Potato

作　　者：徐文瑾 范军亮 李世贵 王凯彤[2,3] 张宁 司怀军[1,2] XU Wenjin;FAN Junliang;LI Shigui;WANG Kaitong;ZHANG Ning;SI Huaijun(College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070,China;State Key Laboratory of Aridland Crop Science/Gansu Agricultural University,Lanzhou,Gansu 730070,China;College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu 730070,China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]省部共建干旱生境作物学国家重点实验室/甘肃农业大学,甘肃兰州730070 [3]甘肃农业大学农学院,甘肃兰州730070

出　　处：《中国马铃薯》2024年第2期97-105,185,共10页Chinese Potato Journal

基　　金：国家重点研发计划项目(2022YFD1602103);甘肃省科技重大专项计划项目(23ZDNA006)。

摘　　要：NF-Y是一种普遍存在于真核生物中的转录因子,通常以NF-YA/B/C异源三聚体的形式发挥作用,其中NF-YA转录因子通过特异性结合DNA启动子区域的CCAAT box,调控下游靶基因表达以应对胁迫。通过前期对马铃薯NF-Ys基因家族的分析,发现沉默马铃薯StNF-YA9基因,马铃薯对干旱胁迫更为敏感。因此,从马铃薯品种‘Atlantic’中克隆了StNF-YA9基因,生物信息学分析表明该基因总长度为1231 bp,CDS区长723 bp。通过构建StNF-YA9亚细胞定位融合表达载体确定其定位在细胞核中;采用qRT-PCR分析该基因组织和逆境胁迫下表达模式,结果表明其在叶片中相对表达量最高,芽中相对表达量最低,受到聚乙二醇(Polyethylene glycol,PEG)和脱落酸(Abscisic acid,ABA)诱导上调表达。研究结果为马铃薯StNF-YA9基因后续功能研究提供理论基础。NF-Y is a transcription factor ubiquitous in eukaryotes,typically functioning as NF-YA/B/C heterotrimers.The NF-YA transcription factor regulates the expression of downstream target genes in response to stress by specifically binding to the CCAAT box in the DNA promoter region.It was discovered that the potato exhibits increased sensitivity to drought stress when the StNF-YA9 gene is silenced through a comprehensive analysis of the potato NF-Ys gene family.In this research,the StNF-YA9 gene was cloned from the potato variety'Atlantic'.Bioinformatics analysis showed that the total length of StNF-YA9 gene was 1231 bp,and the CDS region length was 723 bp.The subcellular localization fusion expression vector of StNF-YA9 was constructed to determine its localization in the nucleus.The expression pattern of StNF-YA9 in potato tissues and under stress was analyzed by qRT-PCR.The results showed that the relative expression of the StNF-YA9 gene was highest in leaves and lowest in buds.The expression was up-regulated by polyethylene glycol(PEG)and abscisic acid(ABA).The results would provide the theoretical basis for subsequent functional studies on the potato StNF-YA9 gene.

关 键 词：马铃薯 StNF-YA9 克隆 生物信息学分析 亚细胞定位 

分 类 号：S532[农业科学—作物学]