基于血细胞全长转录物组和血清差异蛋白质组的厚壳贻贝新型免疫相关蛋白质鉴定  

Novel Immune-related Proteins Identified from Mytilus coruscus by Hemocytes Full-length Transcriptome and Serum Differential Proteome

作　　者：肖文慧 王昊东 阳宗欣 宋方 王月 何建瑜 张晓林 严小军 廖智 XIAO Wen-Hui;WANG Hao-Dong;YANG Zong-Xin;SONG Fang;WANG Yue;HE Jian-Yu;ZHANG Xiao-Lin;YAN Xiao-Jun;LIAO Zhi(Laboratory of Marine Biology Resource and Molecular Engineering,Marine Science and Technical College,Zhejiang Ocean University,Zhoushan 316022,Zhejiang,China)

机构地区：[1]浙江海洋大学海洋科学与技术学院,海洋生物资源与分子工程研究室,浙江舟山316022

出　　处：《中国生物化学与分子生物学报》2024年第7期947-963,共17页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金重点国际合作与交流项目(No.42020104009);国家自然科学基金面上项目(No.32271580)资助。

摘　　要：贻贝是一类极具经济价值和生态价值的双壳贝类,对其先天免疫防御机制的研究在海洋生物免疫学研究中占据重要地位。血淋巴是贻贝的主要免疫组织,采用贻贝血细胞Nanopore全长转录物组,结合基于SDS-PAGE分析的血清差异蛋白质组学手段,开展了厚壳贻贝血淋巴对不同微生物免疫响应的关键分子挖掘。研究结果表明,在不同微生物诱导后的贻贝血清中,共鉴定到蛋白质44种,其中26种蛋白质表现为在不同微生物胁迫后具有与对照组相比的显著差异表达(P<0.05),其功能涉及蛋白质折叠保护、细胞自噬与凋亡的调节、活性氧产生、能量代谢调节、细胞解毒功能以及炎症反应调节等。上述蛋白质在不同细菌和真菌胁迫后,其在血细胞中的基因表达量变化趋势存在差异,反映出贻贝对不同细菌和真菌诱导具有不同的响应策略。上述研究结果为了解贻贝先天免疫系统在应对不同类别微生物侵袭过程中,其差异化免疫响应机制,以及贻贝在微生物感染后,特异性标志蛋白质的筛选提供了新的科学依据,也为后续贝类养殖业的健康发展和病害防治提供了思路。Mytilus is one of bivalves with great economic and ecological values.The innate immune defense of Mytilus shows great significance in the study of marine biological immunology.Hemolymph is the main immune tissue for Mytilus.The Nanopore full-length transcriptome of Mytilus coruscus hemocytes,and the serum differential proteomics based on SDS-PAGE analysis were performed to identify key proteins involving in the immune response of Mytilus hemolymph in response of different bacteria and fungi stresses.A total of 44 proteins were identified in the serum induced by different microorganisms.Among them,26 proteins showed significant differential expression level in response to different microbial stresses,and their functions were involved in protein folding protection,cell autophagy and apoptosis regulation,reactive oxygen species production,energy metabolism regulation,cell detoxification,and immune regulation.The changes in expression levels of these proteins varied in response to different bacterial and fungal stresses,suggesting that Mytilus has different immune response strategies to different bacterial and fungal stresses.The results provide a new scientific basis for understanding the differential immune mechanism of Mytilus innate immune system in response to different types of microbial invasion,as well as the screening of specific biomarker proteins for microbial infection,and provide ideas for the healthy development and disease prevention of shellfish aquaculture.

关 键 词：厚壳贻贝 血淋巴 全长转录物组 差异蛋白质组 免疫 

分 类 号：Q789[生物学—分子生物学]