miR-19a靶向胰岛素样生长因子2受体抑制血管瘤干细胞的增殖和迁移  

MiR-19a affects hemangioma stem cells proliferation and migration by targeting insulin-like growth factor 2 receptor

作　　者：汪帆 吴瑶 方林森[1] 曹东升[2] Wang Fan;Wu Yao;Fang Linsen;Cao Dongsheng(Dept of Wound Repair&Plastic Surgery,The First Affiliated Hospital of Anhui Medical University(Anhui Public Health Clinical Center),Hefei 230001;Dept of Plastic Surgery,The Second Affiliated Hospital of Anhui Medical University,Hefei 230001)

机构地区：[1]安徽医科大学第一附属医院北区(安徽省公共临床中心)创面修复与整形美容外科,合肥230001 [2]安徽医科大学第二附属医院创面修复与整形美容外科,合肥230001

出　　处：《安徽医科大学学报》2024年第6期1029-1034,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽省自然科学基金项目(编号:1808085mh282);安徽医科大学科研基金项目(编号:2019xkj062)。

摘　　要：目的探讨婴儿血管瘤(IHs)中miR-19a是否与胰岛素样生长因子2受体(IGF-2R)相互作用,影响血管瘤干细胞(HemSCs)的增殖、迁移和脂肪生成。方法从IHs标本中分离、筛选和培养HemSCs,免疫组织化学鉴定IGF-2R在HemSCs中表达。用miR-19a模拟物和抑制剂对HemSCs进行转染,通过CCK-8、划痕实验、Transwell、qRT-PCR和免疫印迹相关实验验证miR-19a对于HemSCs增殖与迁移的影响。结果与对照组相比,用miR-19a抑制剂处理的HemSCs增殖与迁移速度显著增加,miR-19a过表达显著抑制IGF-2诱导的细胞迁移和增殖(P<0.05)。结论miR-19a可能通过靶向IGF-2R抑制HemSCs的增殖、迁移和脂肪生成。Objective To investigate whether miR-19a interacts with insulin-like growth factor 2 receptors(IGF-2R)in infantile hemangiomas(IHs)and affects the proliferation,migration,and adipogenesis of hemangioma stem cells(HemSCs).Methods HemSCs were isolated,screened and cultured from IH specimens.IGF-2R expression in HemSCs was identified using immunohistochemistry.HemSCs transfected with miR-19a mimics and inhibitors were subjected to CCK-8,wound healing,Transwell,qRT-PCR,and Western blot analyses.Results Compared with the control,the proliferation and migration rate of HemSCs treated with miR-19a inhibitors were significantly increased,and overexpression of miR-19a significantly inhibited IGF-2 induced cell migration and proliferation(P<0.05).Conclusion MiR-19a may inhibit HemSCs proliferation,migration,and adipogenesis by targeting IGF-2R.

关 键 词：血管瘤干细胞 微小RNA IGF-2R 增殖 迁移 

分 类 号：R732.2[医药卫生—肿瘤]