包载铑纳米颗粒的复合水凝胶对胰腺癌BxPC-3细胞的杀伤效应  

Killing effect of composite hydrogel containing Rhodium nanoparticles on pancreatic cancer BxPC-3 cells

作　　者：王宁薇 张聪 刘刚 余跃 Wang Ningwei;Zhang Cong;Liu Gang;Yu Yue(Division of Gastroenterology,The First Affiliated Hospital of University of Science and Technology of China,Hefei 230001;Dept of Spleen and Stomach,Susong County Traditional Chinese Medicine Hospital,Anqing 246000)

机构地区：[1]中国科学技术大学附属第一医院消化内科,合肥230001 [2]宿松县中医院脾胃病科,安庆246000

出　　处：《安徽医科大学学报》2024年第6期1040-1046,共7页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:31870993)。

摘　　要：目的探究包载铑纳米颗粒的复合水凝胶NPN+Rh-PEG NPs(NRP)对胰腺癌BxPC-3细胞的杀伤效应。方法首先利用原子转移自由基聚合(ATRP)合成嵌段共聚物,再通过水相法合成PEG修饰的铑纳米颗粒,并通过超声混匀制备预混液后升温合成负载纳米颗粒的复合水凝胶NRP。对其进行表征和催化性能验证。利用透射电镜及扫描电镜对铑纳米颗粒和复合水凝胶NRP进行形貌表征。用热成像仪检测复合水凝胶NRP的光热性能,然后用噻唑蓝比色法(MTT)和活-死细胞染色法观察其对BxPC-3细胞的生长抑制作用。最后,使用MTT和血液相容性测试验证其生物安全性。结果成功制备出粒径约为10 nm的铑纳米(Rh-PEG)。在冷冻扫描电镜下复合水凝胶显示多孔结构,铑元素均匀分布在复合水凝胶内部。在激光功率1 W/cm^(2)的808 nm近红外光(NIR)照射下,80μg/ml的NRP生成活性氧(ROS)的能力是纯水凝胶(NPN)的19.6倍(P<0.05);光照条件下,催化过氧化氢分解率高达96.8%。在激光功率1 W/cm^(2)的808 nm NIR照射下,5 min内80μg/ml的NRP能够升温至58.9℃。MTT结果显示,40μg/ml的NRP在1 W/cm^(2)的808 nm NIR照射后,BxPC-3细胞存活率最低,仅为14.8%。活-死细胞染色结果证明,与不使用808 nm NIR照射比较,光照下40μg/ml的NRP的细胞杀伤作用更强。结论均匀包载铑纳米颗粒的复合水凝胶NRP有效地增强对胰腺癌BxPC-3细胞的杀伤效应。Objective To investigate the killing effect of Rhodium nanoparticles loaded composite hydrogel NPN+Rh-PEG NPs(NRP)on pancreatic cancer BxPC-3 cells.Methods Block copolymers were synthesized using atom transfer radical polymerization(ATRP),followed by the synthesis of PEG-modified Rhodium nanoparticles through an aqueous method.A premixed solution was prepared by ultrasonication and then heated to synthesize the composite hydrogel NRP loaded with nanoparticles.It was then characterized and its catalytic properties were verified.The morphology of Rhodium nanoparticles and the composite hydrogel NRP was characterized by transmission electron microscopy and scanning electron microscopy.The thermal imaging instrument was used to detect the photothermal properties of the composite hydrogel NRP,and then the growth inhibitory effect on BxPC-3 cells was observed using the MTT and live-dead staining methods.Finally,its biological safety was verified using MTT and blood compatibility testing.Results Rh-PEG with a particle size of about 10 nm was successfully prepared.The composite hydrogel showed porous structure under cryo scanning electron microscope,and Rhodium was evenly distributed in the composite hydrogel.Under the irradiation of 808 nm near-infrared light(NIR)with a laser power of 1 W/cm^(2),the ability of 80μg/ml NRP to generate reactive oxygen species(ROS)was 19.6 times that of pure hydrogel(NPN)(P<0.05).Under light conditions,the catalytic decomposition rate of hydrogen peroxide was as high as 96.8%.Under the irradiation of 808 nm NIR with a laser power of 1 W/cm^(2),the temperature of 80μg/ml NRP could rise to 58.9℃within 5 minutes.MTT results showed that the survival rate of BxPC-3 cells was the lowest,only 14.8%,after 40μg/ml NRP was irradiated with 1 W/cm^(2) of 808 nm NIR.The results of live dead cell staining proved that the cell killing effect of 40μg/ml NRP under light irradiation was stronger than that without 808 nm NIR irradiation.Conclusion The composite hydrogel NRP uniformly loaded with Rhodiu

关 键 词：铑纳米颗粒 水凝胶 活性氧 胰腺癌 

分 类 号：R735.9[医药卫生—肿瘤]