PPARG rs2290449位点与延边地区脓毒症伴MODS联合用药疗效的关系  

Relationship between PPARG rs2290449 and the efficacy of combination therapy in sepsis with MODS in Yanbian area

作　　者：孙数耀 蔡宛轩 刘霞 金光[1] 崔青松 Sun Shuyao;Cai Wanxuan;Liu Xia;Jin Guang;Cui Qingsong(Laboratory of Pathology and Pathophysiology,School of Medicine,Yanbian University,Yanji 133002;Dept of Critical Care Medicine,Yanbian University Hospital,Yanji 133002)

机构地区：[1]延边大学医学院病理与病理生理实验室,延吉133002 [2]延边大学附属医院重症医学科,延吉133002

出　　处：《安徽医科大学学报》2024年第7期1257-1262,共6页Acta Universitatis Medicinalis Anhui

基　　金：吉林省联合基金白求恩医学专项(编号:20200201413JC)。

摘　　要：目的探讨基因PPARG rs2290449的单核苷酸多态性与脓毒症伴多器官功能障碍综合征(MODS)联合用药疗效的相关性。方法选择382例接受联合用药治疗的脓毒症伴MODS患者,分为有效组(n=192)和无效组(n=190),通过病例对照方法进行研究。利用Sequenom MassARRAY质谱为基础的基因分型方法检测PPARG rs2290449位点的基因型和等位基因分布频率。通过非条件Logistic回归计算比值比(OR)及95%可信区间(CI),评估携带不同基因型患者对脓毒症伴MODS联合用药的疗效。结果PPARG rs2290449位点包括G和A两种等位基因,GG、GA、AA三种基因型;经非条件Logistic回归分析,与携带GG基因型人群比较,携带GA基因型人群与脓毒症伴MODS联合用药疗效有明显相关性(OR=0.449,95%CI=0.280~0.722,P=0.001)。在显性模型中,与携带GG基因型人群比较,携带GA+AA基因型人群与脓毒症伴MODS联合用药疗效有明显相关性(OR=2.104,95%CI=1.332~3.321,P=0.001)。校正混杂因素后,经非条件Logistic回归分析,与携带GG基因型人群比较,携带GA+AA基因型人群与脓毒症伴MODS联合用药疗效有明显相关性(OR=2.307,95%CI=1.438~3.701,P=0.001)。分层分析显示:与携带GG基因型人群比较,在年龄、性别、民族分层分析中,携带GA+AA基因型人群与脓毒症伴MODS联合用药疗效有明显相关性。结论PPARG rs2290449位点单核苷酸多态性与延边地区脓毒症伴MODS联合用药疗效存在明显相关性。Objective To investigate the relationship between the single nucleotide polymorphism of PPARG rs2290449 and the efficacy of combination therapy in sepsis patients with MODS.Methods 382 cases of sepsis with MODS were selected and divided into the effective group and the ineffective group,and a case-control study was conducted.PCR-RFLP and Sequenom MassARRAY were used to detect the genotype and allele frequencies of the loci.Unconditional Logistic regression was used to calculate odds ratio(OR)and 95%confidence interval(CI)to evaluate the efficacy of combination therapy for sepsis with MODS in patients with different genotypes.Results There were G and A alleles in PPARG rs2290449 locus,GG、GA and AA genotype.Non-conditional Logistic regression analysis showed that compared with the GG genotype group,the GA genotype group had a significant correlation with the efficacy of combination therapy for sepsis with MODS(OR=0.449,95%CI=0.280-0.722,P=0.001).In the dominant model,there was a significant correlation between the GA+AA genotype and the efficacy of combination therapy compared with the GG genotype(OR=2.104,95%CI=1.332-3.321,P=0.001).After adjusting for confounding factors,unconditional Logistic regression analysis showed that compared with the GG genotype,the GA+AA genotype was significantly correlated with the efficacy of combination therapy in sepsis patients with MODS(OR=2.307,95%CI=1.438-3.701,P=0.001).Stratified analysis showed that compared with the population carrying GG genotype,the population carrying GA+AA genotype was significantly correlated with the efficacy of combination therapy in sepsis with MODS in the stratified analysis of age,gender and ethnicity.Conclusion PPARG rs2290449 single nucleotide polymorphism is significantly correlated with the efficacy of combination therapy in sepsis patients with MODS in Yanbian area.

关 键 词：脓毒症 单核苷酸多态性 联合用药 多器官功能障碍 

分 类 号：R365[医药卫生—病理学]