大豆磷脂散对神经损伤保护作用的研究  

Study on the protective effect of soybean phospholipid powder on nerve injury

作　　者：周梦丽 饶先玥 方婧 汪浩[1] Zhou Mengli;Rao Xianyue;Fang Jing;Wang Hao(Dept of Oncology Radiotherapy,The First Affiliated Hospital of Anhui Medical University,Hefei 230022)

机构地区：[1]安徽医科大学第一附属医院肿瘤放疗科,合肥230022

出　　处：《安徽医科大学学报》2024年第5期797-802,共6页Acta Universitatis Medicinalis Anhui

基　　金：安徽高校自然科学研究项目(编号:KJ2021A0308)。

摘　　要：目的探讨大豆磷脂散对体外神经细胞、小鼠神经组织的保护作用及其机制。方法细胞实验中,细胞计数试剂盒(CCK-8)法观察不同浓度大豆磷脂散对小鼠小胶质细胞(BV2)和大鼠肾上腺髓质嗜铬瘤细胞(PC12)的细胞毒性作用;NO测定实验分析不同浓度大豆磷脂散对BV2细胞分泌NO水平的影响;显微镜下观察大豆磷脂散对PC12细胞突触生长活性作用;动物实验部分,利用东莨菪碱小鼠模型模拟小鼠认知功能障碍,Morris水迷宫实验测试小鼠学习记忆能力;苏木精-伊红染色(HE)观察东莨菪碱模型小鼠海马组织形态及神经细胞密度。结果大豆磷脂散在浓度为1000μg/ml以下时,对BV2细胞和PC12细胞无明显细胞毒性作用。与对照组相比,大豆磷脂散预处理的BV2细胞NO分泌量明显降低(P<0.01),PC12细胞的神经突生长显著增加(P<0.01)。与模型组相比,大豆磷脂散显著改善东莨菪碱模型小鼠的学习记忆能力(P<0.05),减轻海马齿状回(DG)、锥体细胞区3(CA3)、锥体细胞区1(CA1)神经元损伤,提高神经细胞密度(P<0.001)。结论大豆磷脂散通过从细胞水平上减轻神经炎症、促进神经突生长,改善认知障碍小鼠学习记忆能力,减轻海马组织损伤,发挥神经保护作用。Objective To investigate the protective effects and mechanisms of soybean phospholipid powder on nerve cells in vitro and rats neural tissues.Methods In the cell experiments,the cytotoxicity of soybean phospholipid powder with different concentrations on mouse microglia cells(BV2)and rat adrenal pheochromocytoma(PC12)cells was observed by cell counting kit-8(CCK-8)assay.The effect of soybean phospholipid powder on the NO level of BV2 cells was analyzed by NO determination experiment,and the synaptic growth of PC12 cells was observed under the microscope.In the animal experiment,the cognitive dysfunction of rat was simulated by scopolamine rat model.Then the learning and memory abilities of rat were tested by Morris water maze experiment;hippocampal tissue morphology and nerve cell density of scopolamine model mice were observed by hematoxylin-eosin staining(HE)staining.Results Soybean phospholipid powder had no obvious cytotoxicity on BV2 cells and PC12 cells within the concentration of 1000μg/ml.Compared with the control group,the NO secretion of BV2 cells pretreated with soybean phospholipid powder significantly decreased(P<0.01),and the neuronal synapse growth of PC12 cells significantly increased(P<0.01).In comparison to the model group,soybean phospholipid powder significantly improved the learning and memory ability of scopolamine model rats(P<0.05),reduced the neuronal damage in dentate gyrus(DG),cornu ammonis3(CA3),cornu ammonis1(CA1)areas of hippocampus,and increased the density of nerve cells(P<0.001).Conclusion Soybean phospholipid powder can play a neuroprotective role by reducing neuroinflammation and promoting neuronal synapse growth at the cellular level,and improve the learning and memory ability of rats with cognitive impairment,reduce hippocampal tissue damage.

关 键 词：大豆磷脂散 神经炎症 促神经突生长 学习记忆 海马损伤 神经保护 

分 类 号：R741[医药卫生—神经病学与精神病学]