PKC/TRPV1通路在大鼠三叉神经痛中的病理作用  

Pathological role of PKC/TRPV1 pathway in trigeminal neuralgia in rats

作　　者：吴贝贝 沈莲 王元银[1] 王烈成 Wu Beibei;Shen Lian;Wang Yuanyin;Wang Liecheng(College&Hospital of Stomatology,Anhui Medical University,Anhui Provincial Key Laboratory of Oral Disease Research,Hefei 230032;Dept of Physiology,School of Basic Medicine,Anhui Medical University,Hefei 230032)

机构地区：[1]安徽医科大学口腔医学院,安徽医科大学附属口腔医院,安徽省口腔疾病研究重点实验室,合肥230032 [2]安徽医科大学基础医学院生理学教研室,合肥230032

出　　处：《安徽医科大学学报》2024年第5期846-851,共6页Acta Universitatis Medicinalis Anhui

基　　金：国家自然科学基金(编号:81971236)。

摘　　要：目的探究蛋白激酶C(PKC)/瞬时感受器电位香草酸亚型1(TRPV1)通路在大鼠三叉神经疼痛(TN)中的病理作用。方法采用眶下神经慢性收缩术(ION-CCI)来创建大鼠TN的模型。将大鼠随机分为假手术组(Sham组)、CCI组、CCI+DMSO组、CCI+GF109203X(双吲哚马来酰亚胺,一种PKC抑制剂)组。使用Von Frey毛刷检测大鼠的机械痛阈。采用qRT-PCR及Western blot分别检测三叉神经节(TG)内PKC和TRPV1表达量的变化。HE染色观察TG的病理变化。结果CCI组大鼠机械痛阈降低(P<0.05),大鼠TG内的磷酸化的PKC(p-PKC)和TRPV1表达显著增加(P<0.05)。组织病理学结果显示,与Sham组相比,CCI组观察到TG出现明显的炎性细胞浸润增多、神经细胞肿胀等变化。注射GF109203X后降低了大鼠TG内PKC的磷酸化和TRPV1的表达,大鼠机械痛阈升高(P<0.05),光学显微镜下可见TG内细胞肿胀和炎症细胞有所减少。结论PKC/TRPV1通路可能参与了大鼠的三叉神经痛。Objective To investigate the pathological role of detect protein kinase C(PKC)/transient receptor potential vanilloid subtype 1(TRPV1)pathway in trigeminal neuralgia(TN)in rats.Methods The infraorbital nerve-chronic constriction injury(ION-CCI)was used to establish a rat model of TN.The rats were randomly divided into Sham group,CCI group,CCI+DMSO group and CCI+GF109203X(a PKC inhibitor)group.The mechanical pain threshold of the rats was measured using a Von Frey brush.qRT-PCR and Western blot were used to detect PKC and TRPV1 in the trigeminal ganglion(TG).HE staining was used to observe the pathological changes of TG.Results The mechanical pain threshold significantly decreased(P<0.05),and the expression of phosphorylated PKC(p-PKC)and TRPV1 in TG significantly increased in the CCI group(P<0.05).Histopathological results showed that compared with the Sham group,the CCI group observed significant changes in TG such as increased inflammatory cell infiltration and nerve cell swelling.Injection of GF109203X effectively reduced the phosphorylation of PKC and the expression of TRPV1 in the TG of rats,and the mechanical pain threshold of the rats increased(P<0.05).Under the light microscope,cell swelling and inflammatory cells in the TG were reduced.Conclusion PKC/TRPV1 pathway may be involved in trigeminal neuralgia in rats.

关 键 词：PKC TRPV1 TG 三叉神经痛 机械痛阈 

分 类 号：R745.11[医药卫生—神经病学与精神病学]