TEAD1/TEAD4基因多态性与非贲门胃癌变关系的研究  

Associations of TEAD1/TEAD4 gene polymorphisms with non-cardia gastric carcinogenesis

作　　者：阎小霞 董文杰[1] 张云翔 高芳[2] 贾彦彬[1] Yan Xiaoxia;Dong Wenjie;Zhang Yunxiang;Gao Fang;Jia Yanbin(School of Basic Medicine and Forensic Medicine,Baotou Medical College,Baotou 014040;College of Medical Technology and Anesthesia,Baotou Medical College,Baotou 014040)

机构地区：[1]包头医学院基础医学与法医学院,包头014040 [2]包头医学院医学技术与麻醉学院,包头014040

出　　处：《安徽医科大学学报》2024年第5期863-868,共6页Acta Universitatis Medicinalis Anhui

基　　金：内蒙古自治区自然科学基金(编号:2020LH08004、2021MS08054);内蒙古自治区卫生健康科技计划项目(编号:202201370);包头医学院创新团队发展计划项目(编号:bycxtd-06);包头医学院花蕾计划项目(编号:HL2021034)。

摘　　要：目的探讨TEA转录因子1(TEAD1)基因单核苷酸多态性(SNP)rs2304733、TEA转录因子4(TEAD4)SNPrs7135838和rs1990330与非贲门胃癌变发病风险的关系。方法采用酶联免疫吸附测定法(ELISA)检测正常对照组血清样本中抗幽门螺杆菌(Hp)的特异性抗体,根据抗体滴度将470例正常对照组分为Hp感染阴性组(n=223)和阳性组(n=247)。在450例非贲门胃癌病例组和470例对照组中,采用聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术对各SNP位点进行基因分型,采用非条件性Logistic回归评估各SNP位点与非贲门胃癌变发病风险的关系。结果TEAD1、TEAD4各SNP位点均与Hp感染没有关联;TEAD1 rs2304733与非贲门胃癌的发病风险相关,与携带TT基因型者相比,携带CT基因型及CC基因型者均增加了非贲门胃癌的发病风险(CTvsTT:OR=2.321,95%CI:1.690~3.188;CCvsTT:OR=5.140,95%CI:1.080~24.463);TEAD4 rs1990330与非贲门胃癌发病风险相关,与携带GG基因型者相比,携带GT基因型者增加了非贲门胃癌的发病风险(OR=2.405,95%CI:1.480~3.908);TEAD4 rs7135838与非贲门胃癌的发病风险无关联;TEAD1rs2304733、TEAD4 rs7135838以及rs1990330对非贲门胃癌发病风险存在交互作用(P<0.05)。结论在包头地区汉族人群中,TEAD1 rs2304733、TEAD4 rs1990330在Hp感染中不起主要作用,在非贲门胃癌发病风险中可能起一定作用;TEAD4 rs7135838在Hp感染以及非贲门胃癌发病风险中可能均不起主要作用;TEAD1 rs2304733、TEAD4 rs1990330对非贲门胃癌发病风险的协同效应最强,为最佳交互模型。Objective To investigate the associations of the single nucleotide polymorphism(SNP)rs2304733 in TEA domain transcription factor 1(TEAD1),rs7135838 and rs1990330 in TEA domain transcription factor 4(TEAD4)genes with the risk of non-cardia gastric carcinogenesis.Methods Enzyme linked immunosorbent assay(ELISA)was used to detect specific antibodies against Helicobacter pylori(Hp)in serum samples of the normal control group.470 normal controls were divided into Hp infection negative group(n=223)and positive group(n=247)based on antibody titers.In the 450 non-cardia gastric cancer cases and 470 controls,polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP)was used to genotype the each SNP locus.The unconditional Logistic regression method was used to evaluate the associations between each SNP locus and the risk of non-cardia gastric carcinogenesis.Results The SNPs of TEAD1 and TEAD4 were not associated with Hp infection.TEAD1 rs2304733 was associated with the risk of non-cardia gastric cancer.Compared with the carriers of TT genotype,the carries of CT and CC genotypes had an increased risk of non-cardia gastric cancer(CT vs TT:OR=2.321,95%CI:1.690-3.188;CC vs TT:OR=5.140,95%CI:1.080-24.463).TEAD4 rs1990330 was associated with the risk of non-cardia gastric cancer.Compared with the carriers of GG genotype,those with GT genotype had an increased risk of non-cardia gastric cancer(OR=2.405,95%CI:1.480-3.908).TEAD4 rs7135838 was not associated with the risk of non-cardia gastric cancer.TEAD1 rs2304733,TEAD4 rs7135838 and rs1990330 had interaction effects on the risk of non-cardia gastric cancer(P<0.05).Conclusion In Baotou Han population,TEAD1 rs2304733 and TEAD4 rs1990330 do not play a major role in Hp infection,but may play a role in the risk of non-cardia gastric cancer.TEAD4 rs7135838 may not play a major role in the risk of Hp infection and non-cardia gastric cancer.TEAD1 rs2304733 and TEAD4 rs1990330 have the strongest synergistic effect on the risk of non-cardia gastric cancer,which is the be

关 键 词：TEA转录因子1 TEA转录因子4 基因多态性 幽门螺杆菌 非贲门胃癌 

分 类 号：R735.2[医药卫生—肿瘤]