青少年正畸固定矫治中釉质健康与脱矿状态下龈上菌斑真菌微生物组的比较研究  

Comparative study on supragingival mycobiome between health and white spot lesions state in adolescents undergoing fixed orthodontic therapy

作　　者：马雁崧 杨昊 刘颖[1] 王红梅[1] 谢贤聚[1] 李晓玮[1] 白玉兴[1] MA Yan-song;YANG Hao;LIU Ying;WANG Hong-mei;XIE Xian-ju;LI Xiao-wei;BAI Yu-xing(Department of Orthodontics,Capital Medical University School of Stomatology,Beijing 100050,China)

机构地区：[1]首都医科大学口腔医学院正畸科,北京100050

出　　处：《北京口腔医学》2024年第3期153-158,共6页Beijing Journal of Stomatology

基　　金：国家自然科学基金(82001078);北京市医管局青年人才培养计划“青苗计划”(QML20231504);首都医科大学附属北京口腔医院创新团队建设项目(CXTD202203);北京市医院管理中心临床医学发展专项经费资助(ZLRK202330)。

摘　　要：目的牙釉质脱矿是固定矫治常见的并发症。本研究比较了固定矫治中釉质脱矿与健康状态下龈上菌斑真菌组的差异,探索口腔真菌与正畸釉质脱矿的关系。方法选取54名固定矫治患者,釉质脱矿组31人,健康对照组23人。收集龈上菌斑,通过转录间隔子测序比较两组真菌组差异,使用RT-qPCR分析白色念珠菌的丰度变化。结果两组真菌微生物多样性和优势物种丰度均存在显著差异。脱矿组真菌微生物多样性降低,念珠菌属及白色念珠菌在脱矿组中富集,且白色念珠菌在脱矿组中丰度较高(P<0.05)。结论青少年固定矫治脱矿状态下龈上菌斑真菌组与健康状态存在显著差异,白色念珠菌更易富集在脱矿菌斑中,可能与釉质脱矿的发生发展密切相关。Objective White spot lesions(WSLs)have a high incidence in fixed orthodontic patients.This study aims at exploring the differences in supragingival dental fungal microbiome between health and WSLs state during fixed orthodontic treatment and explore the potential role of fungal microbiome in orthodontic WSLs.Methods 54 patients undergoing fixed orthodontic treatment were selected in this study,including 31 in the WSLs group and 23 in the healthy group.ITS sequencing was used to compare the differences in supragingival fungal microbiome between the two groups,and the absolute abundance of Candida albicans was quantitatively analyzed by RT-qPCR.Results There were significant differences in microbial diversity and abundance of dominant genera between WSLs and the control group.The community diversity of fungal microbiome decreased significantly in the WSLs group,and Candida as well as Candida albicans were significantly enriched in the WSLs group.In addition,the absolute abundance of Candida albicans in the WSLs group was significantly higher than that in the control group(P<0.05).Conclusions Our study elucidated that there are significant differences in the supragingival fungal microbiome between adolescent patients with or without WSLs.Candida albicans is more enriched in demineralized lesions,which may be closely related to the occurrence and development of WSLs in fixed orthodontics.

关 键 词：固定矫治 釉质脱矿 真菌组学 ITS测序 白色念珠菌 

分 类 号：R783.5[医药卫生—口腔医学]