富含羟基酪醇和玉米黄质色素橄榄油对衰老小鼠影响的比较  

Comparative study of the effects of olive oil rich in hydroxytyrosol and zeaxanthin pigment on aging mice

作　　者：叶文斌 陈文东 王昱 何九军 黄新异[2] 邸多隆[2] YE Wenbin;CHEN Wendong;WANG Yu;HE Jiujun;HUANG Xinyi;DI Duolong(College of Agriculture and Forestry Technology,Longnan Normal University Chengxian 742500,China;Key laboratory of Chemistry of North Western Plant Resources&Key Laboratory for Natural Medicine of Gansu Province,Lanzhou Institute of Chemical Physics,Chinese Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]陇南师范学院农林技术学院,甘肃成县742500 [2]中国科学院兰州化学物理研究所中科院西北特色植物资源化学重点实验室,甘肃省天然药物重点实验室,甘肃兰州730000

出　　处：《甘肃农业大学学报》2024年第3期10-16,30,共8页Journal of Gansu Agricultural University

基　　金：甘肃省科技重大专项“高品质橄榄油关键技术开发、集成示范与应用”(21ZD4NK045);甘肃省重点研发计划项目“具有‘明目’和‘降血脂’活性的橄榄油功能食品的研发及生物功能评价”(22YF7FK220);甘肃省高校教师创新基金项目“富含主米黄质色素橄榄油延缓D-半乳糖诱导衰老小鼠的作用研究”(2024B-335)。

摘　　要：【目的】研究富含羟基酪醇橄榄油(olive oil rich in hydroxyl tyrosol,OHT)和富含玉米黄质色素橄榄油(olive oil rich in zeaxanthin,OZT)对衰老小鼠的影响。【方法】利用0.5 mL/10g剂量颈部皮下注射5%的D-半乳糖(D-galactose)溶液42 d构建亚急性衰老模型,建模成功后,将小鼠随机分成5组,分别为模型组(Model group)、维生素E对照组(VE group)、普通橄榄油组(olive oil group,OL)、OHT组和OZT组,以生理盐水对照组CK(0)。连续灌胃28 d后,检测小鼠体质量、血清中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)、丙氨酸氨基转移酶(alanine aminotransferase,ALT)、天门冬酸氨基转移酶(aspartate aminotransferase,AST)活性和丙二醛(malondialdehyde,MDA)、白细胞介素-2(IL-2)、和干扰素-γ(IFN-γ)生成水平等指标的影响,观察肝组织形态学变化。【结果】D-半乳糖诱导的衰老小鼠模型中,OHT和OZT均能控制衰老小鼠体质量(P<0.05),降低血清中的SOD、CAT、GSH-Px、ALT和AST的活性(P<0.05,P<0.01),能显著提高IL-2和IFN-γ生成(P<0.05,P<0.01),降低MDA含量(P<0.05,P<0.01),肝脏病理变化得到改善,OZT干预效果显著优于OHT。【结论】OHT和OZT对D-半乳糖致亚急性衰老动物模型均有很好的干预效果,能有效提高小鼠机体抗氧化能力,延缓衰老的作用。【Objective】 To study the effects of olive oil rich in hydroxyl tyrosol(OHT)and zeaxanthin(OZT) on aging mice.【Method】 A subacute aging model of mice was established by subcutaneous injection of 5% D-galactose solution in the neck at a dose of 0.5 mL/10 g for 42 days,the mice were randomly divided into model group(model group),vitamin E control group(VE group),olive oil group(OL),OHT group and OZT group,normal saline was used as CK(0) group,After continuous gavage for 28 days,to analysis the effects of different doses of OHT and OZT on body weight,serum levels of SOD,CAT,GSHPX,ALT,AST and MDA,IL-2 and IFN-γ in aging mice were analyzed.【Result】 In D-galactose-induced aging mice,OHT and OZT controlled the body weight of aging mice(P<0.05),decreased the activities of SOD,CAT,GSH-Px,ALT and AST in serum(P<0.05,P<0.01),IL-2 and IFN-γ production were significantly increased(P<0.05,P<0.01),the contents of MDA were decreased(P<0.05,P<0.01),The pathological changes of liver were improved,and the effect of OZT was significantly better than that of OHT.【Conclusion】 OHT and OZT have a good therapeutic effect on the subacute aging model induced by D-galactose,which may be attributed to their ability to effectively improve the antioxidant capacity of mice and enhance the effect of delaying aging.

关 键 词：羟基酪醇 玉米黄质色素 橄榄油 衰老小鼠 抗氧化 抗衰老 

分 类 号：TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程]