山奈酚对高糖诱导的人肾上皮细胞增殖与凋亡的影响  

Effects of Kaempferol on Proliferation and Apoptosis of Human Renal Epithelial Cells Under High Glucose

作　　者：喻信林 宋莹[1] 张宇晴[1] 颜伟[1] 周黄燕 邹学森[1] YU Xinlin;SONG Ying;ZHANG Yuqin(Jiangxi Cancer Hospital,Nanchang,330029)

机构地区：[1]江西省肿瘤医院,330029

出　　处：《实用癌症杂志》2024年第7期1100-1104,1114,共6页The Practical Journal of Cancer

基　　金：江西省中医药管理局科技计划项目(编号:2019A158);江西省卫生健康委项目(编号:202310928);江西省肿瘤医院院内科研开放基金项目(编号:KFJJ2023YB03)。

摘　　要：目的 探究山奈酚对高糖诱导人肾上皮细胞(Hkb20)增殖及凋亡的影响。方法 分组培养人肾上皮细胞分为正常糖浓度组(对照组)、高糖组、氯沙坦组(阳性对照)和不同浓度山奈酚组(10μmol/L、30μmol/L、50μmol/L山奈酚与高糖共同作用)。采用CCK-8法和AV/PI法应用流式细胞仪分别检测细胞增殖和凋亡情况,qRT-PCR检测TGF-β1和Smad3 mRNA水平的表达,Western blot法检测细胞因子TGF-β1和Smad3蛋白含量。结果 高糖能诱导人肾上皮细胞增殖降低,使细胞凋亡率升高,增加TGF-β1和Smad3 mRNA和蛋白的表达水平(P<0.05)。不同浓度山奈酚处理后,山奈酚能显著增强细胞增殖和降低细胞凋亡率。同时TGF-β1和Smad3 mRNA和蛋白的表达水平下降,且呈剂量依赖性(P<0.05)。结论 山奈酚可逆转高糖诱导的人肾上皮细胞增殖降低和凋亡增加。这说明山奈酚可能是通过TGF-β1/Smad3信号通路抑制高糖环境下人肾上皮细胞的增殖降低和凋亡增加,从而最终导致肾脏纤维化。Objective To investigate the effects of kaempferol on the proliferation and apoptosis of human renal epithelial cells(Hkb20)induced by high glucose,and to clarify its related mechanisms.Methods Human renal epithelial cells were cultured in groups,including normal glucose group(control group),high glucose group,losartan group(positive control)and different concentrations of kaempferol groups(10μmol/L,30μmol/L,50μmol/L kaempferol and high glucose co-action).The cell proliferation and apoptosis were detected by CCK-8 method and AV/PI method flow cytometry,the expression of TGF-β1 and Smad3 mRNA was detected by qRT-PCR,and the protein content of TGF-β1 and Smad3 was detected by Western blot.Results High glucose could induce the decrease of human renal epithelial cell proliferation,increase the apoptosis rate,and increase the expression levels of TGF-β1 and Smad3 mRNA and protein(P<0.01).After different concentrations of kaempferol treatment,kaempferol could significantly increase cell proliferation and decrease apoptosis rate.At the same time,the expression levels of TGF-β1 and Smad3 mRNA and protein decreased,and showed a dose-dependent manner(P<0.05).Conclusion Kaempferol could reverse the decrease of human renal epithelial cell proliferation and increase of apoptosis induced by high glucose.This suggests that kaempferol may inhibit the decrease of human renal epithelial cell proliferation and increase of apoptosis under high glucose environment through TGF-β1/Smad3 signaling pathway,which can eventually lead to renal fibrosis.

关 键 词：山奈酚 高糖 人肾上皮细胞 TGF-β1/Smad3 纤维化 糖尿病肾病 

分 类 号：R737.11[医药卫生—肿瘤]