肺癌患者放疗前后外周血DC细胞和血清sIL-2R、HMGB-1变化及其与预后的关系研究  

Changes of Peripheral Blood DC Cells and Serum sIL-2R and HMGB-1 Levels in Patients with Lung Cancer Before and After Radiotherapy and Their Clinical Significance

作　　者：杨艳萍 高岭 赵明 弋振营 YANG Yanping;GAO Ling;ZHAO Ming(Kaifeng Central Hospital,Kaifeng,475000)

机构地区：[1]河南省开封市中心医院,475000

出　　处：《实用癌症杂志》2024年第7期1186-1191,共6页The Practical Journal of Cancer

摘　　要：目的 探讨肺癌患者放疗前后外周血DC细胞和血清sIL-2R、HMGB-1水平变化及临床意义。方法 收集肺癌患者124例,采用适形调强放疗(intensity modulated radiation therapy, IMRT)技术治疗,于放疗前、放疗后抽取空腹状态下静脉血4 ml,采用免疫磁珠法检测外周血DC细胞(DC1、DC2)水平,采用双抗体夹心酶联免疫法检测血清sIL-2R、HMGB-1水平。统计不同性别、年龄、是否吸烟、病理类型、是否手术、KPS评分、临床分期的肺癌患者外周血DC细胞和血清sIL-2R、HMGB-1水平,并分析患者预后与血清sIL-2R、HMGB-1水平的关系,单因素和多因素COX回归分析影响肺癌患者预后因素。结果 肺癌患者放疗前后外周血DC细胞水平比较,P>0.05。肺癌患者治疗后血清sIL-2R、HMGB-1水平与治疗前比较,P<0.05。治疗前,血清sIL-2R水平在不同性别、年龄、是否吸烟、病理类型方面比较,P>0.05,在是否手术、不同KPS评分、临床分期、淋巴结转移方面比较,P<0.05;血清HMGB-1水平在不同病理类型方面比较,P>0.05,在不同性别、年龄、是否吸烟、是否手术、不同KPS评分、临床分期、淋巴结转移方面比较,P<0.05;治疗后,血清sIL-2R水平在不同性别、年龄、是否吸烟、病理类型方面比较,P>0.05,在是否手术、不同KPS评分、临床分期、淋巴结转移方面比较,P<0.05;血清HMGB-1水平在不同性别、年龄、是否吸烟、病理类型、是否手术、KPS评分、临床分期、淋巴结转移方面比较,P<0.05。死亡组患者血清sIL-2R、HMGB-1水平均高于生存组(P<0.05)。死亡组DC2低于生存组(P<0.05)。经单因素、多因素COX回归分析显示,临床分期(Ⅲ~Ⅳ期)、淋巴结转移、sIL-2R、HMGB-1水平异常上升、DC2比例下降均是导致肺癌患者预后差的独立危险因素。结论 肺癌患者放疗前后外周血DC细胞无明显变化,但血清sIL-2R、HMGB-1水平治疗后较治疗前均明显降低,其sIL-2R、HMGB-1水平异常上升及DC2Objective To investigate the changes and clinical significance of peripheral blood DC cells and serum sIL-2R and HGMB-1 levels in patients with lung cancer before and after radiotherapy.Methods A total of 124 patients with lung cancer were collected and treated with intensity modulated radiation therapy(IMRT).4 ml of fasting venous blood was extracted before and after radiotherapy,and the levels of peripheral blood DC cells(DC1,DC2)were detected by immunomagnetic beads method.The levels of serum sIL-2R and HMGB-1 were detected by double antibody sandwich enzyme-linked immunoassay.Peripheral blood DC cells and serum sIL-2R and HMGB-1 levels in patients with lung cancer were counted according to gender,age,smoking status,pathological type,operation status,KPS score and clinical stage,and the relationship between patients'prognosis and serum sIL-2R and HMGB-1 levels was analyzed.Univariate and multivariate COX regression analysis was performed to analyze the prognostic factors of patients with lung cancer.Results The level of peripheral blood DC cells in patients with lung cancer before and after radiotherapy was compared,P>0.05.The levels of serum sIL-2R and HMGB-1 in patients with lung cancer after treatment were compared with those before treatment,P<0.05.Before treatment,the level of serum sIL-2R was compared in different gender,age,smoking or not,pathological type(P>0.05),and in operation or not,different KPS score,clinical stage and lymph node metastasis(P<0.05).Serum HMGB-1 levels were compared in different pathological types(P>0.05),and in different gender,age,smoking,surgery,KPS score,clinical stage and lymph node metastasis(P<0.05).After treatment,the level[LM]of serum sIL-2R was compared in different gender,age,smoking or not,pathological type(P>0.05),and in operation or not,different KPS score,clinical stage,lymph node metastasis(P<0.05).Serum HMGB-1 levels were compared in different gender,age,smoking,pathological type,surgery,KPS score,clinical stage,lymph node metastasis,P<0.05.The levels of serum sIL

关 键 词：肺癌 放疗 DC细胞 SIL-2R HMGB-1 临床意义 

分 类 号：R734.2[医药卫生—肿瘤]