Hydrogel-supported poly(L-lactic acid) and polystyrene microsphere-based three-dimensional culture systems for in vitro cell expansion  

作　　者：Huaying Hao Lihong Sun Jiaxuan Chen Jun Liang 

机构地区：[1]State Key Laboratory of Food Nutrition and Safety,Tianjin University of Science&Technology,Tianjin 300457,China [2]College of Light Industry Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China [3]College of Food Science and Engineering,Tianjin University of Science&Technology,Tianjin 300457,China

出　　处：《Frontiers of Materials Science》2024年第2期129-137,共9页材料学前沿（英文版）

基　　金：supported by the National Key Research andDevelopment Program of China(Grant No.2021YFC2101400).

摘　　要：The in vitro expansion of stem cells is important for their application in different life science fields such as cellular tissue and organ repair.An objective of this paper was to achieve static cell culture in vitro through peptide hydrogel-supported microspheres(MSs).The peptides,with their gel-forming properties,microstructures,and mechanical strengths characterized,were found to have good support for the MSs and to be injectable.The internal structures of poly(L-lactic acid)microspheres(PLLA-MSs)and polystyrene microspheres(PS-MSs)made in thelaboratory were observed and statistically analyzed in terms of particle size and pore size,following which the co-cultured MSs with cells were found to have good cell adhesion.In addition,three-dimensional(3D)culturing of cells was performed on the peptide and microcarrier composite scaffolds to measure cell viability and cell proliferation.The results showed that the peptides could be stimulated by the culture medium to self-assembly form a 3D fiber network structure.Under the peptide-Ms composite scaffold-based cell culture system,further enhancement of the cell culture effect was measured.The peptide-Ms composite scaffolds have great potential for the application in 3D cell culture and in vitro cellexpansion.

关 键 词：MICROCARRIER MICROSPHERE peptide hydrogel cell scaffold three-dimensional culture cell expansion 

分 类 号：R318[医药卫生—生物医学工程] TQ427.26[医药卫生—基础医学]