CDCA5基因在子宫颈癌组织细胞中表达变化及对肿瘤细胞增殖的影响  

Expression changes of CDCA5 gene in cervical cancer tissues and cells and its effect on proliferation of cervical cancer cells

作　　者：黄爱敏 刘艳[2] 宋翰 刘晗黎 王希波[2] HUANG Aimin;LIU Yan;SONG Han;LIU Hanli;WANG Xibo(School of Clinical Medicine,Shandong Second Medical University,Weifang 261042,China;不详)

机构地区：[1]山东第二医科大学临床医学院,山东潍坊261042 [2]潍坊市人民医院妇科

出　　处：《山东医药》2024年第20期11-15,共5页Shandong Medical Journal

基　　金：山东省医药卫生科技发展计划项目(202105010306)。

摘　　要：目的 观察细胞分裂周期相关蛋白5(CDCA5)基因在子宫颈癌组织细胞中的表达变化,观察CDCA5基因过表达对子宫颈癌细胞增殖能力的影响,并探讨可能的分子机制。方法 收集子宫颈癌组织和癌旁正常组织各60例,培养人宫颈上皮永生化细胞系H8和人子宫颈癌细胞系SiHa、C-33A、MS751、HeLa,采用实时荧光定量PCR法检测组织和细胞中的CDCA5 mRNA。将CDCA5 mRNA相对表达量最低的子宫颈癌细胞随机分为对照组、过表达组,分别转染pCDH空载体质粒、CDCA5过表达质粒,采用CCK-8实验、克隆形成实验检测细胞增殖能力,Western blotting法检测细胞中的p-ERK、p-AKT蛋白。结果 子宫颈癌组织中CDCA5 mRNA相对表达量高于癌旁组织(P<0.05);子宫颈癌细胞系SiHa、C-33A、MS751、HeLa中CDCA5 mRNA表达高于H8细胞,其中HeLa细胞中相对表达量最低(P均<0.05)。过表达组24、48、72 h细胞增殖能力高于对照组,过表达组细胞平板克隆形成数量及p-ERK、p-AKT蛋白表达高于对照组(P均<0.05)。结论 CDCDA5基因在子宫颈癌组织、细胞中高表达,CDCDA5基因过表达可增强人子宫颈癌细胞的增殖能力,该作用可能与调控ERK/AKT信号通路有关。Objective To observe the expression changes of the CDCA5 gene in the cervical cancer tissues and cells,to observe the effect of its overexpression on the proliferation of cervical cancer cells,and to investigate the potential underlying molecular mechanism.Methods Sixty pairs of cervical cancer tissues and adjacent tissues were obtained for analysis,along with the immortalized human cervical epithelial cell line H8 and human cervical cancer cell lines SiHa,C-33A,MS751,and HeLa,which were cultured for experimentation.The levels of CDCA5 mRNA in both the tissues and cells were assessed using real-time fluorescent quantitative PCR.The cervical cancer cells exhibiting the lowest relative expression of CDCA5 mRNA were randomly assigned into the control and overexpression groups,which were transfected with pCDH empty vector and CDCA5 overexpressed vector,respectively.Cell proliferation was assessed using CCK-8 and clone formation tests,while Western blotting was employed to detect the p-ERK and p-AKT protein.Results The relative expression level of CDCA5 mRNA in the cervical cancer tissues was higher than that in the adjacent tissues(P<0.05);the expression of CDCA5 mRNA in cervical cancer cell lines SiHa,C-33A,MS751,and HeLa was higher than that in H8 cells,among which the relative ex-pression level was the lowest in HeLa cells(all P<0.05).The cell proliferation abilities at 24,48,and 72 h were higher in the overexpression group than in the control group,and the number of cell plate clone formation and the expression of p-ERK and p-AKT proteins in the overexpression group were higher than those in the control group(all P<0.05).Conclusion CDC-DA5 gene is highly expressed in cervical cancer tissues and cells,and the overexpression of CDCDA5 gene can enhance the proliferation of human cervical cancer cells,which may be related to the regulation of ERK/AKT signaling pathway.

关 键 词：细胞分裂周期相关蛋白5 子宫颈癌 细胞增殖 ERK/AKT信号通路 

分 类 号：R737.33[医药卫生—肿瘤]