栉孔扇贝Tssk2基因的序列特征及表达分析  

Sequence Characteristics and Expression Analysis ofTssk 2 Gene in Chlamys farreri

作　　者：邢妍 王春雨 罗冬艳 刘晓玲[1] XING Yan;WANG Chunyu;LUO Dongyan;LIU Xiaoling(School of Life Sciences,Yantai University,Yantai 264005,China)

机构地区：[1]烟台大学生命科学学院,山东烟台264005

出　　处：《烟台大学学报（自然科学与工程版）》2024年第3期293-298,共6页Journal of Yantai University(Natural Science and Engineering Edition)

基　　金：山东省自然科学基金资助项目(ZR2022MC126);山东省贝类产业技术体系项目(SDAIT-14-05)。

摘　　要：为研究栉孔扇贝(Chlamys farreri)睾丸特异性丝氨酸/苏氨酸激酶2基因Tssk 2的序列和表达模式,利用荧光定量PCR及生物信息学分析研究了该基因的序列特征、各组织表达和性腺周期表达,结果显示该基因CDS为723 bp,编码240个氨基酸;进化树显示,栉孔扇贝Tssk2蛋白的进化地位与生物学分类地位一致;栉孔扇贝Tssk2具有Tssk家族的S_TKc保守结构域,含丝氨酸/苏氨酸蛋白激酶活性区域(VIb区域)和ATP结合区,但ATP结合区域缺少Ⅰ子域。Tssk 2在栉孔扇贝各组织和性腺发育周期中的表达显示,精巢是Tssk 2表达量最高的组织,尤其是成熟期精巢中表达量最高,推测Tssk 2在成熟精巢中发挥重要作用,或与精子的成熟相关。In order to study the sequence and expression pattern of testis-specific serine/threonine-protein kinase 2 gene(Tssk 2)in Chlamys farreri,the sequence characteristics,tissue expression and gonadal cycle expression of Tssk 2 gene are studied by qRT-PCR and bioinformatics analysis.The results are as follows:The CDS(coding sequences)of Tssk 2 is 723 bp long and encodes 240 amino acids.Phylogenetic analysis shows that the evolutionary status of Tssk 2 protein in C.farreri is consistent with its biological classification status.Tssk 2 possesses the S_TKc conserved domain of the TSSKs,including the serine/threonine protein kinase active region(VIb region)and ATP-binding region,but the ATP-binding region lacks theⅠsubdomain.The expression of Tssk 2 gene in various tissues and gonadal development cycles of C.farreri show that Tssk 2 expression is the highest in the sperms,especially in the sperms at maturity,which shows that this gene may play a key role in mature testes and/or is relates to sperm maturation.

关 键 词：栉孔扇贝 Tssk 2 序列特征 表达 

分 类 号：S917.4[农业科学—水产科学]