circ_0073585在急性髓系白血病中的表达及功能研究  

Expression and Function of circ_0073585 in Acute Myeloid Leukemia

作　　者：周永勍 苏肖宇 赵倩 邓兆群[1,2] ZHOU Yong-Qing;SU Xiao-Yu;ZHAO Qian;DENG Zhao-Qun(Laborutory Center,The Affiliated People’s Hospital of Jiangsu University,The Key Lab of Precision Diagnosis and Treatment of Zhenjiang City,Zhenjiang 212002,Jiangsu Province,China)

机构地区：[1]江苏大学附属人民医院中心实验室 [2]镇江市精确诊断与治疗重点实验室,江苏镇江212002

出　　处：《中国实验血液学杂志》2024年第4期1018-1025,共8页Journal of Experimental Hematology

基　　金：国家自然科学基金资助项目(81970156)。

摘　　要：目的:研究环状RNA(circular RNA,circRNA)circ_0073585在急性髓系白血病(AML)患者骨髓中的表达水平、临床意义及其在白血病细胞中的功能。方法:采用实时定量PCR(RQ-PCR)检测106例初诊AML患者及38例对照者骨髓标本中circ_0073585的表达水平,比较两组的表达差异并分析其临床意义。根据受试者工作特征(ROC)曲线来评估circ_0073585的表达对AML的诊断价值。将过表达circ_0073585载体及空载体的慢病毒感染的THP-1细胞分为circ_0073585-THP-1和NC-THP-1两组,通过CCK-8法、流式细胞术分析circ_0073585对THP-1细胞增殖、生存、凋亡及药物敏感性的影响。结果:与对照组相比,circ_0073585在AML患者骨髓中的表达水平显著降低(P<0.001)。circ_0073585高、低表达组在白细胞计数(P<0.05)、血小板计数(P<0.01)及染色体危险程度(P<0.05)方面差异有统计意义。与NC-THP-1细胞相比,circ_0073585-THP-1细胞增殖及生存能力减弱(P<0.01)、凋亡率增加(P<0.01),对高三尖杉酯碱(P<0.05)及柔红霉素(P<0.001)的敏感性增加。结论:circ_0073585在AML患者中表达水平下降,过表达circ_0073585可抑制白血病细胞的增殖能力、生存能力,促进凋亡,并提高白血病细胞对化疗药物的敏感性。Objective:To investigate the expression level,clinical significance and function of circular RNAs(circRNAs)circ_0073585 in the bone marrow of patients with acute myeloid leukemia(AML).Methods:The expression levels of circ_0073585 in bone marrow samples of 106 newly diagnosed AML patients and 38 controls were detected by real-time quantitative PCR(RQ-PCR).The differences were compared between the two groups and their clinical significance was analyzed.The diagnostic value of circ_0073585 expression for AML was evaluated by receiver operating characteristic curve(ROC).THP-1 cells with lentivirus overexpressing circ_0073585 vector and empty vector were divided into two groups:circ_0073585-THP-1 and NC-THP-1 group.CCK-8 assay and flow cytometry were used to study the effects of circ_0073585 on THP-1 cell proliferation,survival,apoptosis and drug sensitivity.Results:Compared with the controls,the expression level of circ_0073585 in the bone marrow of AML patients was significantly reduced(P<0.001).There was a statistically significant difference between the high and low expression groups of circ_0073585 in the white blood cell count,platelet count(P<0.01)and chromosome risk(P<0.05).Compared with NC-THP-1 cells,the proliferation and viability of circ_0073585-THP-1 cells were weakened(P<0.01),the apoptosis rate was increased(P<0.01),and the sensitivity to homoharringtonine(P<0.05)and daunorubicin hydrochloride(P<0.001)was increased.Conclusion:The expression level of circ_0073585 is decreased in AML patients.Overexpression of circ_0073585 can inhibit the proliferation and viability of leukemic cells,promote apoptosis,and increase sensitivity of leukemia cells to chemotherapy drugs.

关 键 词：环状RNA circ_0073585 急性髓系白血病 

分 类 号：R733.71[医药卫生—肿瘤]