胶原蛋白肽对模拟微重力条件下鼠T淋巴细胞功能的改善作用  

Effect of Collagen Peptides on Function of Mouse T Lymphocytes under Simulated Microgravity

作　　者：司少艳 秦亚亚 吴莹莹 马小淯 尚颖 宋淑军 郭燕川[2] SI Shao-Yan;QIN Ya-Ya;WU Ying-Ying;MA Xiao-Yu;SHANG Ying;SONG Shu-Jun;GUO Yan-Chuan(Deparment of Comprehensive Basic Experiment(Division Two),Srategic Suppont Force Medical Center,Bejjing 100101,China;Technical Institute of Physics and Chemistry,Chinese Academy of Sciences,Key Laboratory of Photochemical Comversion and Optoelec-tromic Materials,Beijing 100190,China)

机构地区：[1]战略支援部队特色医学中心综合基础实验研究室(研究部二室),北京100101 [2]中国科学院理化技术研究所,光化学转换与功能材料重点实验室,北京100190

出　　处：《中国实验血液学杂志》2024年第4期1258-1263,共6页Journal of Experimental Hematology

基　　金：战略支援部队特色医学中心课题(19ZX22)。

摘　　要：目的:了解胶原蛋白肽对模拟微重力条件下培养的鼠T淋巴细胞功能的影响。方法:分离小鼠的脾细胞,采用旋转式细胞培养系统模拟微重力条件,采用有丝分裂源刀豆球蛋白A刺激T淋巴细胞,并分别加入不同浓度的胶原蛋白肽,检测淋巴细胞的增殖能力及培养上清中细胞因子的水平。结果:模拟微重力可以使脾脏T淋巴细胞的增殖能力受到抑制,产生细胞因子的水平下降,而胶原蛋白肽能促进模拟微重力条件下培养的淋巴细胞增殖及细胞因子分泌。结论:胶原蛋白肽可能通过调节淋巴细胞的增殖功能及细胞因子的分泌,减轻模拟微重力环境下对T淋巴细胞的抑制作用。Objective:To understand the effect of collagen peptides on the function of mouse lymphocytes under simulated microgravity.Methods:The splenocytes of mice were isolated,and the rotary cell culture system was used to simulate the microgravity.The T lymphocytes were stimulated with mitotic agents,concanavalin A(ConA),and the cells were treated with different concentrations of collagen peptides.The proliferation of lymphocytes and the levels of cytokines in the supernatant were detected.Results:Simulated microgravity could inhibit the proliferation of spleen T lymphocytes and decrease the level of cytokines in the supernatant.Collagen peptides could promote the lymphocyte proliferation and cytokine production in cells cultured under simulated microgravity.Conclusion:Collagen peptides may attenuate the inhibitory effect of simulated microgravity on T lymphocytes by regulating the cell proliferation and the secretion of cytokines.

关 键 词：胶原蛋白肽 模拟微重力 淋巴细胞 细胞增殖 细胞因子 

分 类 号：R392.12[医药卫生—免疫学]