Single-cell and spatial omics:exploring hypothalamic heterogeneity  

作　　者：Muhammad Junaid Eun Jeong Lee Su Bin Lim 

机构地区：[1]Department of Biochemistry&Molecular Biology,Ajou University School of Medicine,Suwon,South Korea [2]Department of Biomedical Sciences,Graduate School of Ajou University,Suwon,South Korea [3]Department of Brain Science,Ajou University School of Medicine,Suwon,South Korea

出　　处：《Neural Regeneration Research》2025年第6期1525-1540,共16页中国神经再生研究（英文版）

基　　金：supported by the Korea Health Technology R&D Project through the Korea Health Industry Development Institute (KHIDI);the Ministry of Health&Welfare,Republic of Korea (HR22C1734);the National Research Foundation (NRF) of Korea (2020R1A6A1A03043539,2020M3A9D8037604,2022R1C1C1004756)(to SBL);the NRF of Korea (2022R1C1C1005741 and RS-2023-00217595);the new faculty research fund of Ajou University School of Medicine (to EJL)。

摘　　要：Elucidating the complex dynamic cellular organization in the hypothalamus is critical for understanding its role in coordinating fundamental body functions. Over the past decade, single-cell and spatial omics technologies have significantly evolved, overcoming initial technical challenges in capturing and analyzing individual cells. These high-throughput omics technologies now offer a remarkable opportunity to comprehend the complex spatiotemporal patterns of transcriptional diversity and cell-type characteristics across the entire hypothalamus. Current single-cell and single-nucleus RNA sequencing methods comprehensively quantify gene expression by exploring distinct phenotypes across various subregions of the hypothalamus. However, single-cell/single-nucleus RNA sequencing requires isolating the cell/nuclei from the tissue, potentially resulting in the loss of spatial information concerning neuronal networks. Spatial transcriptomics methods, by bypassing the cell dissociation, can elucidate the intricate spatial organization of neural networks through their imaging and sequencing technologies. In this review, we highlight the applicative value of single-cell and spatial transcriptomics in exploring the complex molecular-genetic diversity of hypothalamic cell types, driven by recent high-throughput achievements.

关 键 词：cellular diversity HYPOTHALAMUS multi-omics single-cell transcriptomics spatial transcriptomics 

分 类 号：Q42[生物学—神经生物学]