Mechanism by which Rab5 promotes regeneration and functional recovery of zebrafish Mauthner axons  

作　　者：Jiantao Cui Yueru Shen Zheng Song Dinggang Fan Bing Hu 

机构地区：[1]Center for Advanced Interdisciplinary Science and Biomedicine of IHM,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei,Anhui Province,China

出　　处：《Neural Regeneration Research》2025年第6期1816-1824,共9页中国神经再生研究（英文版）

基　　金：supported by the Research Funds of the Center for Advanced Interdisciplinary Science and Biomedicine of IHM,No.QYZD20220002;the National Natural Science Foundation of China,No.82071357;a grant from the Ministry of Science and Technology of China,No.2019YFA0405600 (all to BH)。

摘　　要：Rab5 is a GTPase protein that is involved in intracellular membrane trafficking. It functions by binding to various effector proteins and regulating cellular responses, including the formation of transport vesicles and their fusion with the cellular membrane. Rab5 has been reported to play an important role in the development of the zebrafish embryo;however, its role in axonal regeneration in the central nervous system remains unclear. In this study, we established a zebrafish Mauthner cell model of axonal injury using single-cell electroporation and two-photon axotomy techniques. We found that overexpression of Rab5 in single Mauthner cells promoted marked axonal regeneration and increased the number of intra-axonal transport vesicles. In contrast, treatment of zebrafish larvae with the Rab kinase inhibitor CID-1067700markedly inhibited axonal regeneration in Mauthner cells. We also found that Rab5 activated phosphatidylinositol 3-kinase(PI3K) during axonal repair of Mauthner cells and promoted the recovery of zebrafish locomotor function. Additionally, rapamycin, an inhibitor of the mechanistic target of rapamycin downstream of PI3K, markedly hindered axonal regeneration. These findings suggest that Rab5 promotes the axonal regeneration of injured zebrafish Mauthner cells by activating the PI3K signaling pathway.

关 键 词：axonal regeneration Mauthner cell nerve regeneration Rab5 ZEBRAFISH 

分 类 号：Q42[生物学—神经生物学]