减压水蒸气蒸馏提取绿橙皮挥发油及其体外抗氧化活性  

A study on extraction of volatile oil from green orange peel by reduced-pressure steam distillation and in vitro antioxidant activity

作　　者：李桂娟 赵会平 肖叶芝 甘文程 范思琪 LI Guijuan;ZHAO Huiping;XIAO Yezhi;GAN Wencheng;FAN Siqi(School of Chemistry and Materials Engineering Hainan Vocational University of Science and Technology,Haikou,Hainan 571126,China;Applied Chemistry Research Center of Hainan Vocational University of Science and Technology,Haikou,Hainan 571126,China)

机构地区：[1]海南科技职业大学化学与材料工程学院,海南海口571126 [2]海南科技职业大学应用化学研究中心,海南海口571126

出　　处：《食品与机械》2024年第6期199-205,共7页Food and Machinery

基　　金：海南省自然科学基金(编号:521RC1154)。

摘　　要：[目的]建立一种减压水蒸气蒸馏提取绿橙皮挥发油的方法。[方法]将挥发油测定器与真空装置组合进行绿橙皮挥发油提取,真空度由装置中的调节阀控制,通过单因素和正交试验,确立减压条件下挥发油提取最佳工艺,利用气相色谱—质谱联用技术(GC-MS)测定减压与常压条件下挥发油组分和含量,并对挥发油体外抗氧化活性进行评价。[结果]减压水蒸气蒸馏提取挥发油最佳工艺为:物料粒度8目,固液比(m绿橙皮∶V水)1∶7(g/mL),真空度0.082 MPa,时间40 min,该条件下挥发油得率为1.769 mL/100 g。减压水蒸气蒸馏提取的绿橙皮挥发油中鉴定出163种成分,比常压水蒸气蒸馏法多出33种,且主要成分含量提高。减压提取的挥发油对超氧阴离子自由基和羟自由基的清除能力显著高于常压水蒸气蒸馏方法提取的。[结论]采用减压水蒸气蒸馏法提取挥发油具有温度低、时间短、得率高、操作简单、可操作性强的特点,所得挥发油具有较好的抗氧化作用,该方法适于热敏性物质提取分离。[Objective]This study aimed to develop a method for extracting volatile oil from green-orange peel by reduced-pressure steam distillation.[Methods]A volatile oil tester was combined with a vacuum device for the extraction of volatile oil from green-orange peel,and a regulating valve in the device controlled the vacuum level.The composition and content of volatile oil under reduced pressure and atmospheric pressure were determined by gas chromatography-mass spectrometry(GC-MS),and the in vitro antioxidant activity was evaluated.[Results]The optimum process for the extraction of volatile oil by reduced-pressure steam distillation was:particle size of 8 mesh,solid-liquid ratio of 1∶7(g/mL),vacuum of 0.082 MPa,time of 40 min,yield of 1.769 mL/100 g.163 kinds of components were identified in the volatile oil of green orange peel extracted by reduced-pressure steam distillation,which was 33 kinds more than that by atmospheric pressure steam distillation,and the content of major components was increased.The scavenging ability of the volatile oil extracted under vacuum to superoxide anion free radical and hydroxyl free radical was significantly higher than that extracted by atmospheric steam distillation.[Conclusion]The reduced-pressure steam distillation volatile oil extraction has the characteristics of low temperature,short time,high yield,simple operation,and high operability.The obtained volatile oil has a good antioxidant effect,and the method is suitable for the extraction and separation of heat-sensitive substances.

关 键 词：挥发油 绿橙皮 减压水蒸气蒸馏法 抗氧化活性 

分 类 号：TQ645.1[化学工程—精细化工] O657.63[理学—分析化学]