双斑长跗萤叶甲雌虫、雄虫触角转录组及差异表达基因分析  

Analysis of the antennal transcriptome and differentially expressed genes of female and male Monolepta signata

作　　者：何婉洁 孟涵颖 支梦婷 陈静[1] HE Wanjie;MENG Hanying;ZHI Mengting;CHEN Jing(Key Laboratory for Oasis Agricultural Pest Management and Plant Protection Resource Utilization/Agricultural College of Shihezi University,Shihezi Xinjiang 832003,China)

机构地区：[1]石河子大学农学院/新疆绿洲农业病虫害治理与植保资源利用重点实验室,新疆石河子832003

出　　处：《新疆农业科学》2024年第4期984-995,共12页Xinjiang Agricultural Sciences

基　　金：新疆维吾尔自治区重点研发任务专项(2022B02043-2);国家自然科学基金项目(31960541,31460473);新疆生产建设兵团指导性科技计划项目(2022ZD010)。

摘　　要：【目的】建立双斑长跗萤叶甲成虫触角转录组数据库,筛选雌虫、雄虫触角中的差异表达基因并解析其涉及的生理功能,为进一步研究双斑长跗萤叶甲的基因功能分析及嗅觉感受机制奠定分子基础。【方法】采用Illumina高通量测序技术对双斑长跗萤叶甲雌虫、雄虫触角进行转录组测序,对获得的unigenes进行功能注释、通路富集等生物信息学分析,利用RT-qPCR技术检测气味结合蛋白基因在触角中的表达水平。【结果】双斑长跗萤叶甲雌、雄成虫触角转录组测序共获得73050条unigenes,Q30碱基百分比均在93.23%以上,34233条unigenes至少在1个数据库中获得注释。与双斑长跗萤叶甲雌虫触角相比,雄虫触角中检测到395个差异表达基因,其中288个基因上调表达,107个基因下调表达。【结论】在GO注释库中,差异表达基因富集最显著的功能为气味结合、嗅觉受体活性和嗅觉感知。气味结合蛋白基因在雌虫、雄虫触角中的相对表达水平趋势与转录组测序结果相符合,获得了双斑长跗萤叶甲成虫触角转录组数据库。【Objective】To establish the transcriptome database of adult Monolepta signata antennae,screen the differentially expressed genes in the antennae of female and male M.signata and analyze the physiological functions of these genes.【Methods】In this study,Illumina high-throughput sequencing technology was used to conduct transcriptome sequencing on the antennae of male and female M.signata and analyze the functional annotation and pathway enrichment of unigenes,and after that the expression level of odorant binding protein genes in antennae was detected by RT-qPCR.【Results】A total of 73,050 unigenes were obtained from the antennae transcriptome sequencing of female and male of adult M.signata,with the percentage of Q30 bases above 93.23,and 34,233 unigenes were annotated in at least one database(46.86%).A total of 395 differentially expressed genes were detected in the antennae of male M.signata compared with that of female M.signata.Among them,288 genes were up-regulated and 107 genes were down-regulated.【Conclusion】In the GO database,the most significant function of enrichment of these differentially expressed genes were odorant binding,olfactory receptor activity,and sensory perception of smell.RT-qPCR results showed that the relative expression levels of odorant binding protein genes in antennae of female and male M.signata were consistent with the transcriptome sequencing results.The transcriptome database of the antenna of adult M.signata is successfully obtained.

关 键 词：双斑长跗萤叶甲 触角转录组 功能注释 差异表达基因 

分 类 号：S433[农业科学—农业昆虫与害虫防治]