应用脐带血单个核细胞修复小鼠皮肤缺损创伤的实验研究  

Umbilical Cord Blood Mononuclear Cells in the Repair of Skin Defects in Trauma Mice

作　　者：冯伟珂 黄承凤[1] 曾俊权 胡云[1] 张守华[1] 李科浩 陶强[1] FENG Wei-ke;HUANG Cheng-feng;ZENG Jun-quan;HU Yun;ZHANG Shou-hua;LI Ke-hao;TAO Qiang(Department of General Surgery,Jiangxi Provincial Children's Hospital,Nanchang 330038,China;Department of Hematology,the Affiliated Hospital of Jinggangshan University,Ji’an 343600,China)

机构地区：[1]江西省儿童医院普外科,南昌330038 [2]井冈山大学附属医院血液科,吉安343600

出　　处：《南昌大学学报（医学版）》2024年第3期20-25,共6页Journal of Nanchang University：Medical Sciences

基　　金：国家自然科学基金(81460118);江西省卫健委科技计划(20195584,202311081,202310853);江西省研究生创新专项资金项目(YC2022-s224)。

摘　　要：目的 探讨创面局部应用脐带血单个核细胞(UCB-MNCs)治疗皮肤缺损的效果及相关机制。方法 选取45只C57小鼠建立创面损伤模型后分为3组。实验组:先将0.5 mL的UCB-MNCs(细胞数:1×10^(9) mL^(-1))皮下于8~10 mm均匀注射在每只小鼠伤口周围,再将0.5 mL的UCB-MNCs均匀注射在创面表面,共1次;阳性对照组:创口表面涂抹牛碱性成纤维细胞生长因子外用凝胶(商品名:贝复新);空白对照组:不做处理,伤口自然暴露。各组小鼠造模后第0、3、7及14天,采用Elisa技术检测血清内皮生长因子(VEGF)、转化生长因子-β(TGF-β)和碱性成纤维细胞生长因子(bFGF),HE和MASSON染色观察创面组织病理学情况。结果 所有小鼠均造模成功,与空白对照组和阳性对照组相比,实验组在造模后第3、7、14天血清VEGF、TGF-β和bFGF的表达水平显著升高(P<0.05)。术后第14天HE和MASSON染色结果显示实验组小鼠的创伤已全部愈合,且表皮修复状况良好,可以观察到部分炎症细胞的浸润;阳性对照组的小鼠部分表皮修复状况良好,但仍有一部分创伤没有完全愈合,肉芽组织的生长状况良好;空白对照组未完全愈合的创伤周围瘢痕组织增生明显,且出现明显的炎症细胞浸润以及部分创伤仍未愈合的现象。结论 UCB-MNCs修复小鼠的皮肤损失创伤,是通过促进细胞因子的分泌来促进皮肤缺损创伤的愈合。Objective To investigate the efficacy and related mechanisms of traumatic topical application of umbilical cord blood mononuclear cells(UCB-MNCs)in the treatment of skin defects.Methods 45 C57 mice were selected to establish a traumatic injury model and then divided into three groups.In the experimental group,each mouse was first injected with 0.5 mL of UCB-MNCs(cell number:1×10^(9) mL^(-1))subcutaneously every 8-10 mm uniformly around the wound,and then 0.5 mL of UCB-MNCs was uniformly injected on the surface of the wound for one time.In the positive control group,recombinant bovine basic fibroblast growth factor topical gel(trade name:befusine gel)was applied on the wound surface.In the blank control group,no treatment was done and the wound was exposed naturally.Serum endothelial growth factor(VEGF),transforming growth factor-β(TGF-β)and basic fibroblast growth factor(bFGF)were detected by Elisa technique on the 0th,3rd,7th and 14th days after modeling in each group,and the histopathology of the wounds was observed by HE and MASSON staining.Results All mice were successfully modeled,and the expression levels of serum VEGF,TGF-β,and bFGF were significantly higher in the experimental group on the 3rd,7th,and 14th days after modeling compared with those in the blank and positive control groups(P<0.05).The HE and MASSON staining results on the 14th day after the operation showed that the wound in the experimental group of mice was completely healed,the epidermis was well repaired,and some inflammatory cells were infiltrated.In the positive control group,some of the wounds in the mice had good epidermal repair,but some of the wounds had not completely healed,and the growth of granulation tissues was good.In the blank control group,marked proliferation of scar tissue could be seen around incompletely healed wounds,and there was an obvious infiltration of inflammatory cells as well as some wounds that remained unhealed.Conclusion UCB-MNCs could repair skin loss in the trauma mice by promoting the secretion of cyt

关 键 词：脐带血单个核细胞 创面愈合 血管内皮生长因子 转化生长因子-Β 碱性成纤维细胞生长因子 动物 实验 小鼠 

分 类 号：R-332[医药卫生]