富血小板纤维蛋白对牙龈成纤维细胞生物学功能的影响  

Effects of Platelet-rich Fibrin on Biological Function of Gingival Fibroblasts

作　　者：王玉花 柴纪华 张好建 WANG Yuhua;CHAI Jihua;ZHANG Haojian(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,Key Laboratory of Oral Biomedicine Ministry of Education,Hubei Key Laboratory of Stomatology,School&Hospital of Stomatology,Wuhan University,Wuhan 430079,China)

机构地区：[1]口颌系统重建与再生全国重点实验室,口腔生物医学教育部重点实验室,口腔医学湖北省重点实验室,武汉大学口腔医(学)院,湖北武汉430079

出　　处：《口腔医学研究》2024年第7期611-616,共6页Journal of Oral Science Research

摘　　要：目的:探究冷冻干燥富血小板纤维蛋白(freeze-dried platelet-rich fibrin, FD-PRF)对人牙龈成纤维细胞(human gingival fibroblasts, hGFs)功能的影响以及其抗菌性能,为口腔黏膜炎的防治提供新思路。方法:采用流式细胞术、CCK-8检测细胞增殖(cell counting kit-8,CCK-8)、细胞划痕和Transwell实验,检测FD-PRF对hGFs细胞周期、增殖和迁移的影响;采用倾注平板法和振荡培养法研究FD-PRF的抗菌作用。结果:CCK-8实验结果表明,随着FD-PRF浓度升高,hGFs的细胞数量显著增加。细胞周期结果显示,S-G2/M期的细胞比例:5%FD-PRF组>1%FD-PRF组>对照组,而G0-G1期细胞则相反。迁移实验结果显示,FD-PRF显著提升了hGFs的迁移速度。抗菌实验结果显示,FD-PRF组相比于对照组的大肠杆菌和金黄色葡萄球菌数量明显减少。结论:FD-PRF促进hGFs进入细胞分裂期,进而促进细胞增殖。FD-PRF促进hGFs迁移。此外,本研究还证实FD-PRF具有一定的抗菌性。提示FD-PRF可以促进口腔黏膜炎的愈合。Objective:To explore the effects of freeze-dried platelet-rich fibrin(FD-PRF)on human gingival fibroblasts(hGFs)and antibacterial property of FD-PRF,and to provide a new way for controlling refractory oral mucositis(OM).Methods:Cell counting kit-8(CCK-8),flow cytometry,scratch wound,and Transwell assays were used to investigate the effects of FD-PRF on hGFs’cycle,proliferation,and migration.Pour plate and shaking culture methods were explored to investigate the antibacterial ability of FD-PRF.Results:CCK-8 assay showed that hGFs number increased significantly with the increase of FD-PRF concentration.In cell cycle results,proportions of cells at S-G2/M phase:5%FD-PRF group>1%FD-PRF group>control group.And proportions of cells at G1-G0 phase were the opposite.Cells migration assays suggested that FD-PRF improved migration rate of hGFs.For antibacterial function tests of FD-PRF,it was found that clones of E.coli and S.aureus were both reduced in the FD-PRF group.Conclusion:By facilitating hGFs entering the division stage,FD-PRF promotes proliferation of hGFs.FD-PRF possesses significant chemotaxis toward hGFs.Besides,FD-PRF possesses intrinsic antibacterial property.FD-PRF may promote the healing of oral mucositis.

关 键 词：冷冻干燥富血小板纤维蛋白 成纤维细胞 抗菌性 口腔黏膜炎 

分 类 号：R78[医药卫生—口腔医学]