转导蛋白β样1X连接受体1表达对卵巢癌A2780细胞增殖和迁移的影响  

Effect of the expression of transducer proteinβ-like 1 X linked receptor 1 on proliferation and migration of ovarian cancer A2780 cells

作　　者：褚秀 金蔚[2] CHU Xiu;JIN Wei(School of Medicine,Jiangsu University,Zhenjiang Jiangsu 212013;Department of Obstetrics and Gynecology,Changshu Second People′s Hospital,Changshu Jiangsu 215500,China)

机构地区：[1]江苏大学医学院,江苏镇江212013 [2]常熟市第二人民医院妇产科,江苏常熟215500

出　　处：《江苏大学学报（医学版）》2024年第4期331-337,共7页Journal of Jiangsu University：Medicine Edition

基　　金：常熟市卫生和计划生育委员会科技计划立项项目(csws201801);常熟市科技局立项项目(20170016)。

摘　　要：目的:研究转导蛋白β样1X连接受体1(transducin beta-like 1X-linked receptor,TBL1XR1)在卵巢癌患者组织中表达,及其对卵巢癌A2780细胞增殖和迁移的影响。方法:采用实时荧光定量PCR(qRT-PCR)检测10对卵巢癌组织、癌旁组织中及人卵巢癌IOSE80、A2780、CP70、SKOV-3中TBL1XR1 mRNA表达,筛选TBL1XR1 mRNA高表达细胞株。选择4~6周龄雌性BALB/C裸鼠,建立卵巢癌人源肿瘤异种移植(patient-derived tumor xenografts,PDTX)模型;将10只模型鼠均分为siR-NC组和si-TBL1XR1组,每组5只,分别给予siR-NC、si-TBL1XR1局部注射,10 mg/kg,每3 d注射1次,18 d后取各组瘤组织,计算其体积与重量。取卵巢癌A2780细胞,将其分为siR-NC组、si-TBL1XR1组、pcDNA3.1组和pcDNA3.1-TBL1XR1组,分别予以siR-NC、si-TBL1XR1、pcDNA3.1空载质粒和pcDNA3.1-TBL1XR1质粒处理;采用蛋白免疫印迹法检测各组卵巢癌细胞周期蛋白表达,MTT比色法检测细胞活力,流式细胞术检测细胞周期和凋亡细胞比例,以及Transwell细胞迁移实验检测细胞迁移能力。结果:卵巢癌组织中TBL1XR1 mRNA表达明显高于癌旁组织(P<0.05);人卵巢癌A2780细胞系TBL1XR1 mRNA表达明显高于卵巢癌IOSE80、CP70、SKOV-3细胞系(P<0.05)。与siR-NC组相比,第18天si-TBL1XR1组瘤体积明显减小(P<0.05),重量明显降低(P<0.05)。与siR-NC组相比,si-TBL1XR1组促癌细胞周期蛋白表达明显降低(P<0.05),与pcDNA3.1组相比,pcDNA3.1-TBL1XR1组表达则明显升高(P<0.05);与siR-NC组相比,si-TBL1XR1组卵巢癌细胞迁移数明显降低(P<0.05),早期凋亡和晚期凋亡细胞比例明显升高(P<0.05);与pcDNA3.1组相比,pcDNA3.1-TBL1XR1组卵巢癌细胞迁移数明显增多(P<0.05),早期凋亡和晚期凋亡细胞比例明显降低(P<0.05)。结论:TBL1XR1在卵巢癌组织中呈高表达,降低TBL1XR1 mRNA表达可抑制卵巢癌A2780细胞增殖和迁移。Objective:To investigate the expression of transducer protein β-like 1 X linked receptor 1(TBL1XR1)in the tissues of ovarian cancer patients and its effect on the proliferation and migration of ovarian cancer A2780 cells.Methods:Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the expression of TBL1XR1 mRNA in 10 pairs of ovarian cancer tissues and adjacent tissues,and IOSE80,A2780,CP70 and SKOV-3 human ovarian cancer cell lines,the latter was used to screen the cell lines with high expression of TBL1XR1 mRNA.BALB/C nude mice aged 4-6 weeks were selected to establish patient-derived tumor xenografts(PDTX)model.Ten PDTX model mice were divided into siR-NC group and si-TBL1XR1 group,with 5 mice in each group;siR-NC and si-TBL1XR1 were given via local injection,respectively,10 mg/kg,once every 3 days.After 18 days,cancer tissues were harvested from each group,and their volume and weight were calculated.Ovarian cancer A2780 cells were taken and divided into siR-NC group,si-TBL1XR1 group,pcDNA3.1 group and PCDNA3.1-TBL1XR1 group;and they were treated with siR-NC,si-TBL1XR1,empty vector plasmid pcDNA3.1 and PCDNA3.1-TBL1XR1 plasmid,respectively.The expression of cyclin in ovarian cancer cells in each group was detected by Western blotting,the cell viability was detected by MTT,the cell cycle and the proportion of apoptotic cells were detected by flow cytometry,and the cell migration ability was detected by Transwell assay.Results:The expression of TBL1XR1 mRNA in ovarian cancer tissues was significantly higher than that in adjacent tissues(P<0.05),and the expression of TBL1XR1 mRNA in human ovarian cancer A2780 cell line was significantly higher than that in IOSE80,CP70 and SKOV-3 cell lines(P<0.05).Compared with siR-NC group,the tumor volume and weight of si-TBL1XR1 group were significantly decreased on the 18th day(both P<0.05).Compared with the siR-NC group,the expression of cyclin in si-TBL1XR1 group was significantly decreased(P<0.05),while the expression in PCDNA3.1-TBL1XR1 group was significant

关 键 词：卵巢癌 转导蛋白β样1X连接受体1(TBL1XR1) 人源肿瘤异种移植模型 细胞周期 

分 类 号：R737.31[医药卫生—肿瘤]