Coat protein of rice stripe virus enhances autophagy activity through interaction with cytosolic glyceraldehyde-3-phosphate dehydrogenases,a negative regulator of plant autophagy  

作　　者：Wanying Zhao Li Wang Lipeng Li Tong Zhou Fei Yan Heng Zhang Ying Zhu Ida Bagus Andika Liying Sun 

机构地区：[1]State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Northwest A&F University,Yangling 712100,Shaanxi,China [2]Institute of Plant Protection,Jiangsu Academy of Agricultural Sciences,Nanjing 210095,China [3]Institute of Plant Virology,Ningbo University,Ningbo 312362,China [4]State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products,Institute of Virology and Biotechnology,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China [5]College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,China

出　　处：《Stress Biology》2023年第1期35-51,共17页逆境生物学（英文）

基　　金：supported in part by the National Natural Science Foundation of China(32170163)to LS;The program of introducing Talents of Innovative discipline to universities(project 111)(B18042)to LS;The state key laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agroproducts(project 2021DG700024-KF202210)to HZ.

摘　　要：Viral infection commonly induces autophagy,leading to antiviral responses or conversely,promoting viral infection or replication.In this study,using the experimental plant Nicotiana benthamiana,we demonstrated that the rice stripe virus(RSV)coat protein(CP)enhanced autophagic activity through interaction with cytosolic glyceraldehyde-3-phosphate dehydrogenase 2(GAPC2),a negative regulator of plant autophagy that binds to an autophagy key factor,autophagy-related protein 3(ATG3).Competitive pull-down and co-immunoprecipitation(Co-IP)assays showed that RSV CP activated autophagy by disrupting the interaction between GAPC2 and ATG3.An RSV CP mutant that was unable to bind GAPC2 failed to disrupt the interaction between GAPC2 and ATG3 and therefore lost its ability to induce autophagy.RSV CP enhanced the autophagic degradation of a viral movement protein(MP)encoded by a heterologous virus,citrus leaf blotch virus(CLBV).However,the autophagic degradation of RSV-encoded MP and RNA-silencing suppressor(NS3)proteins was inhibited in the presence of CP,suggesting that RSV CP can protect MP and NS3 against autophagic degradation.Moreover,in the presence of MP,RSV CP could induce the autophagic degradation of a remorin protein(NbREM1),which negatively regulates RSV infection through the inhibition of viral cell-to-cell movement.Overall,our results suggest that RSV CP induces a selective autophagy to suppress the antiviral factors while protecting RSV-encoded viral proteins against autophagic degradation through an as-yet-unknown mechanism.This study showed that RSV CP plays dual roles in the autophagy-related interaction between plants and viruses.

关 键 词：RSV Coat protein AUTOPHAGY GAPC ATG3 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]