Effects of perinatal stress on the metabolites and lipids in plasma of dairy goats  

作　　者：Yan Huang Yezi Kong Bowen Li Chenxu Zhao Juan J.Loor Panpan Tan Yang Yuan Fangyuan Zeng Xiaoyan Zhu Simeng Qi Baoyu Zhao Jianguo Wang 

机构地区：[1]College of Veterinary Medicine,Northwest A&F University,Yangling 712100,Shaanxi,China [2]LipidALL Technologies Company Limited,Changzhou 213022,Jiangsu,China [3]Department of Animal Sciences,Division of Nutritional Sciences,University of Illinois,Urbana,IL 61801,USA

出　　处：《Stress Biology》2023年第1期126-144,共19页逆境生物学（英文）

基　　金：supported by the National Natural Science Foundation of China(grant numbers 32273085,32102742).

摘　　要：Dairy goats experience metabolic stress during the peripartal period,and their ability to navigate this stage of lactation is related to the occurrence and development of metabolic diseases.Unlike dairy cows,there is a lack of comprehensive analysis of changes in the plasma profiles of peripartal dairy goats,particularly using high-throughput techniques.A subset of 9 clinically-healthy dairy goats were used from a cohort of 96 primiparous Guanzhong dairy goats(BCS,2.75±0.15).Blood samples were collected at seven time points around parturition(d 21,14,7 before parturition,the day of kidding,and d 7,14,21 postpartum),were analyzed using untargeted metabolomics and targeted lipidomics.The orthogonal partial least squares discriminant analysis model revealed a total of 31 differential metabolites including p-cresol sulfate,pyruvic acid,cholic acid,and oxoglutaric acid.The pathway enrichment analysis identified phenylalanine metabolism,aminoacyl-tRNA biosynthesis,and citrate cycle as the top three significantly-altered pathways.The Limma package identified a total of 123 differentially expressed lipids.Phosphatidylserine(PS),free fatty acids(FFA),and acylcarnitines(ACs)were significantly increased on the day of kidding,while diacylglycerols(DAG)and triacylglycerols(TAG)decreased.Ceramides(Cer)and lyso-phosphatidylinositols(LPI)were significantly increased during postpartum period,while PS,FFA,and ACs decreased postpartum and gradually returned to antepartum levels.Individual species of FFA and phosphatidylcholines(PC)were segregated based on the differences in the saturation and length of the carbon chain.Overall,this work generated the largest repository of the plasma lipidome and metabolome in dairy goats across the peripartal period,which contributed to our understanding of the multifaceted adaptations of transition dairy goats.

关 键 词：Dairy goat Transition period Untargeted metabolomics LIPIDOMICS 

分 类 号：S827[农业科学—畜牧学]