Exploring the precision redox map during fasting-refeeding and satiation in C.elegans  

作　　者：Xinhua Qiao Lu Kang Chang Shi Aojun Ye Dongli Wu Yuyunfei Huang Minghao Deng Jiarui Wang Yuzheng Zhao Chang Chen 

机构地区：[1]National Laboratory of Biomacromolecules,CAS Center for Excellence in Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]School of Basic Medical Sciences of Southwest Medical University,Luzhou 646000,China [3]University of Chinese Academy of Sciences,Beijing 100049,China [4]School of Pharmacy,East China University of Science and Technology,Shanghai 200237,China

出　　处：《Stress Biology》2023年第1期187-200,共14页逆境生物学（英文）

基　　金：supported by the National Key R&D Program(2022YFA1303000 and 2017YFA0504000);the National Natural Science Foundation of China(91849203,31900893);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDB39000000).

摘　　要：Fasting is a popular dietary strategy because it grants numerous advantages,and redox regulation is one mecha-nism involved.However,the precise redox changes with respect to the redox species,organelles and tissues remain unclear,which hinders the understanding of the metabolic mechanism,and exploring the precision redox map under various dietary statuses is of great significance.Twelve redox-sensitive C.elegans strains stably expressing genetically encoded redox fluorescent probes(Hyperion sensing H_(2)O_(2) and Grx1-roGFP2 sensing GSH/GSSG)in three organelles(cytoplasm,mitochondria and endoplasmic reticulum(ER))were constructed in two tissues(body wall muscle and neurons)and were confirmed to respond to redox challenge.The H_(2)O_(2) and GSSG/GSH redox changes in two tissues and three organelles were obtained by confocal microscopy during fasting,refeeding,and satiation.We found that under fasting condition,H_(2)O_(2) decreased in most compartments,except for an increase in mitochondria,while GSSG/GSH increased in the cytoplasm of body muscle and the ER of neurons.After refeeding,the redox changes in H_(2)O_(2) and GSSG/GSH caused by fasting were reversed in most organelles of the body wall muscle and neurons.In the sati-ated state,H_(2)O_(2) increased markedly in the cytoplasm,mitochondria and ER of muscle and the ER of neurons,while GSSG/GSH exhibited no change in most organelles of the two tissues except for an increase in the ER of muscle.Our study systematically and precisely presents the redox characteristics under different dietary states in living animals and provides a basis for further investigating the redox mechanism in metabolism and optimizing dietary guidance.

关 键 词：Redox FASTING REFEEDING SATIATION HYPERION Grx1-roGFP2 Caenorhabditis elegans 

分 类 号：R15[医药卫生—营养与食品卫生学]