羟基红花黄色素A对实验性自身免疫性肝炎小鼠的保护作用  

Protective effect of hydroxylsafflor yellow A on the mice with experimental autoimmune hepatitis

作　　者：彭雪莹 王阳阳[2] 陈治[2] 张建光 李慧 张佳庆 刘英华 PENG Xueying;WANG Yangyang;CHEN Zhi;ZHANG Jianguang;LI Hui;ZHANG Jiaqing;LIU Yinghua(Department of Endoscopy and Minimally Invasive Therapy,Central Hospital of Cangzhou,Cangzhou 061000,China;Endoscopy Center,Affiliated Hospital of Hebei University,Baoding 071000,China;Health Sciences Center of Hebei University,Baoding 071000,China;Emergency Center for Public Health Emergencies of Chengde,Chengde 067000,China)

机构地区：[1]沧州市中心医院内镜微创诊疗科,河北沧州061000 [2]河北大学附属医院内镜中心,河北保定071000 [3]河北大学医学部,河北保定071000 [4]承德市突发公共卫生事件应急中心,河北承德067000

出　　处：《医学研究与教育》2024年第3期1-7,共7页Medical Research and Education

基　　金：2023年河北省自然科学基金面上项目(H2023201022);2022年政府资助临床医学优秀人才培养项目(361007)。

摘　　要：目的 利用刀豆蛋白A(concanavalin A,Con A)诱导的肝损伤小鼠模型,研究羟基红花黄色素A(hydroxylsafflor yellow A,HSYA)对实验性自身免疫性肝炎小鼠的保护作用。方法 36只BALB/c小鼠随机分为6组,Sham组、Con A组、HSYA低剂量组(5 mg/kg)、HSYA中剂量组(10 mg/kg)、HSYA高剂量组(20 mg/kg)、Sham+HSYA高剂量组(20 mg/kg),每组小鼠各6只。将各组小鼠称质量并记录,通过腹腔注射给药的方式对其进行3 d HSYA预处理,处死小鼠前12 h,尾静脉注射Con A,制作实验性自身免疫性肝炎小鼠模型,最终以二氧化碳吸入法处死小鼠并取材备用。使用全自动生化分析仪检测各组小鼠血浆AST和ALT水平,并进行组间比较。肝组织进行苏木精-伊红染色,镜下观察肝脏组织学变化。应用ELISA检测血浆中TNF-α、 IL-17、IL-10水平。结果 Con A组小鼠体内TNF-α、 IL-17、IL-10水平(F=21.19、F=13.12、F=3.209,P均<0.05)及ALT、AST水平(F=274.33、F=214.91,P均<0.05)高于Shan组,并伴有肝细胞大片坏死。HSYA中剂量和HSYA高剂量组小鼠体内炎症因子水平及氨基转移酶水平低于ConA组,同时伴肝细胞坏死区域缩小。结论 Con A成功诱导小鼠肝组织损伤,HSYA对实验性自身免疫性肝炎小鼠具有保护作用。Objective To investigate the protective effect of hydroxylsafflor yellow A(HSYA)on the mice with experimental autoimmune hepatitis using concanavalin A Con A-induced liver injury mouse model.Methods A total of 36 BALB/c mice were randomly divided into Sham group,Con A group,HSYA low-dose group(5 mg/kg),HSYA medium-dose group(10 mg/kg),HSYA high-dose group(20 mg/kg)and Sham+HSYA high-dose group(20 mg/kg),with 6 mice in each group.Mice in each group were weighed and the weight was recorded.Then,the mice were pretreated with HSYA through intraperitoneal injection for three days.The experimental autoimmune mouse model was made by tail intravenous injection of Con A 12 hours before we killed the mice.Mice were killed by carbon dioxide inhalation ultimately and we took materials for the next experimental operations.The AST and ALT levels of each group were detected with automatic biochemical analyzer and compared between groups.The liver tissue were stained with hematoxylin-eosin,we observed the pathological changes of liver tissue under a microscope.The level of IL-17,IL-10 and TNF-αin plasma were measured with enzyme linked immunosorbent assay.Results Compared with the Sham group,Plasma level of TNF-α,IL-17,IL-10 and AST,ALT in mice were significantly increased at 12 hours after Con A treatment(P<0.05),meanwhile a lot of necrotic liver cells could be seen under the microscope.Compared with the Con A group,plasma level of inflammatory factors and transaminase in HSYA(10 mg/kg and 20 mg/kg)group were markedly decreased,meanwhile a few of necrotic liver cells could be seen under the microscope.Conclusion Con A could induce liver injury in mice successfully,HSYA has a protective effect on the mice with experimental autoimmune hepatitis.

关 键 词：羟基红花黄色素A 刀豆蛋白A 白细胞介素-17 白细胞介素-10 肿瘤坏死因子-Α 

分 类 号：R575.1[医药卫生—消化系统]