IS607 TnpB is a hypercompact RNA-guided DNA endonuclease  

作　　者：Hui Yang Dinshaw J.Patel 

机构地区：[1]Key Laboratory of RNA Innovation,Science and Engineering,Shanghai Institute of Biochemistry and Cell Biology,Center for Excellence in Molecular Cell Science,Chinese Academy of Sciences,University of Chinese Academy of Sciences,Shanghai,China. [2]Structural Biology Program,Memorial Sloan-Kettering Cancer Center,New York,NY,USA

出　　处：《Cell Research》2024年第7期473-474,共2页细胞研究（英文版）

摘　　要：The Zhiwei Huang lab report on a hypercompact IS607 TnpB protein that naturally co-occurs with the conserved RAGATH-18 RNA,thereby identifying a new family of compact RNAguided DNA endonucleases.Such an IS607 TnpB-based approach for programmable genome editing in both Escherichia coli and human cells,combined with its structural and target-adjacent motif divergence from earlier reported IS200/IS605 TnpB systems,highlights the diversity of the non-Cas genome editor toolbox.Transposable elements(TEs)are mobile DNA sequences capable of moving around or generating copies of themselves in a host organism’s genome.The IS200/IS605 and IS607 transposons constitute the simplest mobile elements and are widespread in prokaryotes.These transposons typically contain subterminal left end(LE)and right end(RE)palindromic elements,tnpA and tnpB genes,and encode a non-coding RNA transcribed from the transposon right-end element,called right-end RNA(reRNA)orωRNA1,2(Fig.1a,i).For IS200/IS605 transposon,TnpA transposase binds to both the LE and RE elements and catalyzes the cleavage and rejoining of DNA substrates,mediating‘Peel-and-Paste’transposition of single-stranded DNA(ssDNA)1,2(Fig.1a).By contrast,IS607 transposons encode a serine recombinase TnpA for the insertion of double-stranded DNA(dsDNA).

关 键 词：thereby EDITOR INSERTION 

分 类 号：Q2[生物学—细胞生物学]