81个铁线莲品种的SSR指纹图谱构建与遗传多样性分析  

Construction of SSR Fingerprints and Genetic Diversity Analysis of 81 Clematis Cultivars

作　　者：张金炜[1] 林立[1] 张椿芳[1] 雷冬阳 许君 Zhang Jinwei;Lin Li;Zhang Chunfang;Lei Dongyang;Xu Jun(College of Landscape and Ecology,Ningbo City College of Vocational Technology,Ningbo,315100)

机构地区：[1]宁波城市职业技术学院景观生态学院,宁波315100

出　　处：《分子植物育种》2024年第14期4630-4640,共11页Molecular Plant Breeding

基　　金：宁波市科技局农业重大专项(2016C11011)资助。

摘　　要：为揭示铁线莲品种资源的遗传多样性,探索分子标记鉴定方法,以81个铁线莲栽培品种为材料,利用SSR荧光标记毛细管电泳技术进行DNA指纹图谱的构建和遗传关系分析。结果表明:筛选出的10对SSR引物共扩增出129个等位基因,平均等位基因数为12900个;平均有效等位基因数(Ne)、观测杂合度(Ho)、期望杂合度(He)分别为4.663、0.498、0.713;Shannon's信息指数(I)在0.470~2.255之间,平均值为1.758;多态性信息含量(PIC)介于0.251~0.862之间,平均PIC值为0.687;遗传多样性指数(H)变幅在0.296~0.880之间,平均值为0.713,表明铁线莲品种的资源遗传多样性水平较高。基于NJ聚类分析结果表明,供试品种被分为4个类群,与Structure群体遗传结构分析和PCoA分析结果基本一致。此外,利用筛选的SSR引物构建了81个铁线莲品种的特异指纹代码和指纹图谱二维码。本研究结果为铁线莲品种资源的分类、鉴定、保存与利用提供理论依据。In order to reveal the genetic characteristics and explore the molecular identification methods of Clematis variety resources,the capillary electrophoresis detection with fluorescent SSR markers method was used to establish the DNA fingerprints and to analyze the genetic relationship of 81 Clematis varieties.The results showed that 10 pairs of SSR primers were screened out and amplified 129 alleles,with an average of 12900 alle les;the average values fo effective number of alleles(Ne),observed heterozygosity(Ho)and expected heterozygosity(He)were 4.663,0.498 and 0.713,respectively.The Shannon's information index(I)ranged from 0.470~0.255,with an average of 0.687;the polymorphic information content(PIC)at different locus varied from 0.251~0.862 with an average of 0.687;the Nei's gene diversity index(H)ranged from 0.296~0.880 with an average of 0.713,suggesting that there is a high level of polymorphism in Clematis variety resources.Further,the result of NJ cluster analysis based on genetic distance showed that 81 Clematis germplasms were divided into four groups,which was highly consistent with the results of principal coordinate analysis(PCoA)and population genetic structure analysis.Additionally,DNA fingerprints of 81 Clematis varieties were constructed by 10 pairs of SSR primers.The results of this study provide theoretical basis for the classification,identification,preservation and utilization of clematis species resources.

关 键 词：铁线莲 SSR分子标记 遗传多样性 指纹图谱 

分 类 号：S685.99[农业科学—观赏园艺]