低温胁迫对小粒种咖啡幼苗光合及叶绿素荧光特性的影响  

Effects of Low Temperature on Photosynthetic and Chlorophyll Fluorescence Parameters of Coffea arabica

作　　者：黄丽芳[1] 龙宇宙[1] 李金芹 王曦奥 董云萍[1] 秦晓威[1] 王晓阳[1] 孙燕[1] 王宪文 闫林[1] Huang Lifang;Long Yuzhou;Li Jinqin;Wang Xi'ao;Dong Yunping;Qin Xiaowei;Wang Xiaoyang;Sun Yan;Wang Xianwen;Yan Lin(Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops of Hainan Province,Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops(Ministry of Agriculture and Rural Affairs),Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Science,Wanning,571533;College of Tropical Crops,Yunnan Agricultural University,Puer,665099;Baoshan Comprehensive Inspection Cener of Quality and Technical Supervision,Baoshan,678000)

机构地区：[1]中国热带农业科学院香料饮料研究所,农业农村部香辛饮料作物遗传资源利用重点实验室,海南省热带香辛饮料作物遗传改良与品质调控重点实验室,万宁571533 [2]云南农业大学热带作物学院,普洱665099 [3]保山市质量技术监督综合检测中心,保山678000

出　　处：《分子植物育种》2024年第14期4706-4714,共9页Molecular Plant Breeding

基　　金：海南省自然科学基金项目(322QN405);国家现代农业产业技术体系项目(CARS—11);云南省科技厅院士专家工作站项目(202205AF150080)共同资助。

摘　　要：为探讨小粒种咖啡的耐寒机制,以1年生小粒种咖啡苗为试材,设置8℃、4℃、0℃、-2℃4个低温胁迫处理,研究不同温度对其植株、叶片光合参数和叶绿素荧光参数的影响。结果表明:随着温度的降低和处理时间的延长,小粒种咖啡植株寒害程度不断加剧,叶片叶绿素含量、光合参数的Pn、Gs、Tr、WUE、Ls以及叶绿素荧光参数的ΦPSII、qP和ETR整体呈先升后降,Ci、Fo是上升趋势,Fv/Fm则逐渐降低。相关性分析表明,Chl、Pn、WUE、Ls、ETR、qP、qN等指标对咖啡抗寒起正向作用,Ci、Tr与其他指标呈负相关,起负作用。综上所述,低温胁迫抑制了小粒种咖啡苗期叶片光合作用及削弱叶绿素荧光特性,该研究结果为筛选咖啡优异抗寒种质提供参考,并为开展抗寒分子机理研究提供理论依据。To investigate the mechanism of cold-resistance in Coffea arabica,one-year-old Coffea arabica plants were treated by four different low temperatures of 8℃,4℃,0℃,-2℃were used to study the effects of photosynthetic parameters and chlorophyll fluorescence parameters in leaves.The results showed that the degree of chilling injury of Coffea arabica plants is increasing with the decrease of temperature and the extension of treatment time,chlorophyll(Chl)content,net photosynthetic rate(Pn),stomatal conductance(Gs),transpiration rate(Tr),water use efficiency(WUE),stomatal limit value(Ls)of photosynthetic parameters,and actual photochemical quantum yield(ΦPSII),photochemical quenching coefficient(qP)and quantum transfer rate(ETR)of chlorophyll fluorescence parameters as a whole were declined after rising.The intercellular CO2 concentration(Ci)and initial fluorescence(Fo)are increasing,while the maximal photochemical yield(Fv/Fm)decreases gradually.Correlation analysis showed that Chl,Pn,WUE,Ls,ETR,qP and qN of coffea was positive correlated with cold resistance,but the negative correlation between the Ci,Tr and the others.The chlorophyll fluorescence characteristics of sorghum seedlings were weakened by low-temperature stress treatment.The results would provide reference for the screening excellent cold resistant germplasm and resistance molecular mechanism research of coffee.

关 键 词：小粒种咖啡 低温胁迫 光合 叶绿素荧光 

分 类 号：S571.2[农业科学—作物学]