Helicobacter pylori infection alters gastric microbiota structure and biological functions in patients with gastric ulcer or duodenal ulcer  

作　　者：Ling-Xiao Jin Yu-Peng Fang Chen-Mei Xia Teng-Wei Cai Qian-Qian Li Yu-Yin Wang Hai-Fan Yan Xia Chen 

机构地区：[1]Department of Gastroenterology,Wenling Hospital of Wenzhou Medical University(The First People’s Hospital of Wenling),Wenling 317500,Zhejiang Province,China

出　　处：《World Journal of Gastroenterology》2024年第24期3076-3085,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by Wenling Science and Technology Program,China,No.2020S0180101;Science and Technology Program of Traditional Chinese Medicine in Zhejiang Province,China,No.2023ZL784.

摘　　要：BACKGROUND Helicobacter pylori(H.pylori)infection is closely associated with gastrointestinal diseases.Our preliminary studies have indicated that H.pylori infection had a significant impact on the mucosal microbiome structure in patients with gastric ulcer(GU)or duodenal ulcer(DU).AIM To investigate the contributions of H.pylori infection and the mucosal microbiome to the pathogenesis and progression of ulcerative diseases.METHODS Patients with H.pylori infection and either GU or DU,and healthy individuals without H.pylori infection were included.Gastric or duodenal mucosal samples was obtained and subjected to metagenomic sequencing.The compositions of the microbial communities and their metabolic functions in the mucosal tissues were analyzed.RESULTS Compared with that in the healthy individuals,the gastric mucosal microbiota in the H.pylori-positive patients with GU was dominated by H.pylori,with signi-ficantly reduced biodiversity.The intergroup differential functions,which were enriched in the H.pylori-positive GU patients,were all derived from H.pylori,particularly those concerning transfer RNA queuosine-modification and the synthesis of demethylmenaquinones or menaquinones.A significant enrichment of the uibE gene was detected in the synthesis pathway.There was no significant difference in microbial diversity between the H.pylori-positive DU patients and healthy controls.CONCLUSION H.pylori infection significantly alters the gastric microbiota structure,diversity,and biological functions,which may be important contributing factors for GU.

关 键 词：Helicobacter pylori Gastric ulcer Duodenal ulcer Metagenomic sequencing Transfer RNA queuosine-modification Menaquinones 

分 类 号：R573[医药卫生—消化系统]