Effect and mechanism of Qingre Huashi decoction on drug-resistant Helicobacter pylori  

作　　者：Miao-Miao Lin Shan-Shan Yang Qiu-Yue Huang Guang-Hui Cui Xiao-Fen Jia Yao Yang Zong-Ming Shi Hui Ye Xue-Zhi Zhang 

机构地区：[1]Department of Integrated Traditional Chinese and Western Medicine,Peking University First Hospital,Beijing 100034,China [2]Institute of Integrated Traditional Chinese and Western Medicine,Peking University,Beijing 100034,China

出　　处：《World Journal of Gastroenterology》2024年第24期3086-3105,共20页世界胃肠病学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China,No.81973615 and No.82304930;Natural Science Foundation of Beijing,No.7332323;Capital’s Funds for Health Improvement and Research,No.CF2022-2-40711.

摘　　要：BACKGROUND Helicobacter pylori(HP),the most common pathogenic microorganism in stomach,can induce inflammatory reactions in the gastric mucosa,causing chronic gastritis and even gastric cancer.HP infection affects over 4.4 billion people globally,with a worldwide infection rate of up to 50%.The multidrug resistance of HP poses a serious challenge to eradication.It has been monstrated that compared to bismuth quadruple therapy,Qingre Huashi decoction(QHD)combined with triple therapy exhibits comparable eradication rates but with a lower incidence of adverse reactions;in addition,QHD directly inhibit and kill HP in vitro.METHODS In this study,12 HP strains were isolated in vitro after biopsy during gastroscopy of HP-infected patients.In vitro,the minimum inhibitory concentration(MIC)values for clinical HP strains and biofilm quantification were determined through the E-test method and crystal violet staining,respectively.The most robust biofilm-forming strain of HP was selected,and QHD was evaluated for its inhibitory and bactericidal effects on the strain with strong biofilm formation.This assessment was performed using agar dilution,E-test,killing dynamics,and transmission electron microscopy(TEM).The study also explored the impact of QHD on antibiotic resistance in these HP strains with strong biofilm formation.Crystalline violet method,scanning electron microscopy,laser confocal scanning microscopy,and(p)ppGpp chromatographic identification were employed to evaluate the effect of QHD on biofilm in strong biofilm-forming HP strains.The effect of QHD on biofilm and efflux pump-related gene expression was evaluated by quantitative polymerase chain reaction.Non-targeted metabolomics with UHPLC-MS/MS was used to identify potential metabolic pathways and biomarkers which were different between the NC and QHD groups.RESULTS HP could form biofilms of different degrees in vitro,and the intensity of formation was associated with the drug resistance of the strain.QHD had strong bacteriostatic and bactericidal effects on H

关 键 词：Qingre Huashi decoction Helicobacter pylori Drug resistance BIOFILM Metabolomics 

分 类 号：R573[医药卫生—消化系统]