灰树花醇提物化学成分及其生物活性研究  

Chemical Constituents and Bioactivity of Alcohol Extracts from Grifola frondosa

作　　者：何君强 熊雯宇 黄莹[1] 刘斌[1,2] HE Junqiang;XIONG Wenyu;HUANG Ying;LIU Bin(College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China;National Engineering Research Center of JUNCAO Technology,Fuzhou 350002,China)

机构地区：[1]福建农林大学食品科学学院,福建福州350002 [2]国家菌草工程技术研究中心,福建福州350002

出　　处：《食品与生物技术学报》2024年第5期54-63,共10页Journal of Food Science and Biotechnology

基　　金：福建省科技计划重大专项项目(2021NZ029009);福建省科学技术厅区域发展项目(2020N3002);福建省科学技术厅对外合作项目(2021I0008);中国博士后科学基金项目(2020M671918)。

摘　　要：为探明灰树花发挥活性作用的化学成分及其抗氧化活性和对肿瘤细胞的抑制作用,该文分析了灰树花醇提物不同极性溶剂萃取物的主要化学成分及其对1,1-二苯基-2-三硝基苯肼(DPPH)自由基、2,2’-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基和羟自由基的清除能力,以及对肝癌细胞(HepG2)和肺癌细胞(A549)的抑制作用。结果显示,乙酸乙酯萃取物和正丁醇萃取物的抗氧化活性最佳,总酚和黄酮的质量分数与抗氧化活性显著正相关(P<0.05);石油醚萃取物和乙酸乙酯萃取物对肿瘤细胞抑制效果最好,三萜和甾醇质量分数与肿瘤细胞抑制作用极显著正相关(P<0.01)。利用超高效液相色谱质谱联用技术(UPLC-MS)从灰树花醇提物中共鉴定出15个化合物,其中芹菜素和表儿茶素可能是灰树花发挥抗氧化作用的主要化学成分,麦角甾醇和过氧化麦角甾醇可能是发挥肿瘤细胞抑制作用的甾体类化合物。该研究结果可为灰树花作为功能食品进一步开发提供理论依据。To elucidate the bioactive chemical components of Grifola frondosa and their antioxidant and tumor cell inhibition effects,the present study analyzed the main chemical components of ethanol extracts of Grifola frondosa extracted with various polar solvents,as well as their scavenging abilities to 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),and hydroxyl radicals.The inhibitory effects on human hepatocellular carcinoma cells(HepG2),human lung adenocarcinoma cells(A549)were also investigated.The results showed that ethyl acetate extract and n-butanol extract had the highest antioxidant activity,and the mass fractions of total phenols and flavonoids were significantly positively correlated with antioxidant activity(P<0.05).The petroleum ether extract and ethyl acetate extract showed the best inhibitory effect on tumor cells,and the mass fractions of triterpenoids and sterols showed a highly significant positive correlation with tumor cell inhibition(P<0.01).Ultrahigh performance liquid chromatography-mass spectrometry(UPLC-MS)identified a total of 15 compounds from the ethanol extracts of Grifola frondosa,among which apigenin and epicatechin might be the main chemical components responsible for the antioxidant activity of Grifola frondosa,while the steroid compounds exhibiting inhibitory effects on tumor cells might be ergosterol and ergosterol endoperoxide.The results could provide a theoretical basis for the further development of Grifola frondosa as the functional food.

关 键 词：灰树花 不同极性溶剂 化学成分 抗氧化活性 肿瘤细胞抑制作用 

分 类 号：Q71[生物学—分子生物学]