HPLC法同时测定植物饮料中7种绿原酸  

Simultaneous Determination of Seven Chlorogenic Acids in Plant Vegetable Beverages by HPLC

作　　者：黄柳霞 许雪儿 李莲 HUANG Liuxia;XU Xueer;LI Lian(Guangdong Provincial Public Laboratory of Food Industry,Guangzhou 511446,China;Guangdong Food Industry Institute Co.Ltd.,Guangzhou 511446,China;Guangdong Quality Supervision Food Inspection Station,Guangzhou 511446,China)

机构地区：[1]广东省食品工业公共实验室,广东广州511446 [2]广东省食品工业研究所有限公司,广东广州511446 [3]广东省质量监督食品检验站,广东广州511446

出　　处：《海南师范大学学报（自然科学版）》2024年第2期218-223,共6页Journal of Hainan Normal University（Natural Science）

基　　金：广东省食品质量安全服务工程技术研究中心(粤科函产字[2020]141号)。

摘　　要：建立一种同时测定植物饮料中7种绿原酸的HPLC法:采用C18色谱柱,以乙腈+0.1%甲酸+0.1%乙酸作为流动相进行梯度洗脱,检测波长为327 nm,参比波长400 nm。7种绿原酸检出限为0.25 mg/L,在0.5~20μg/mL浓度范围内成线性关系,新绿原酸、绿原酸、隐绿原酸、异绿原酸B、1,5-二咖啡酰奎尼酸、异绿原酸A、异绿原酸C回收率分别为99.0%~108%、97.9%~101%、96.9%~102%、96.2%~106%、95.1%~96.7%、94.9%~99.6%、96.3%~108%。该方法简单、快捷、定性定量准确,可同时测定植物饮料中7种绿原酸。An HPLC method was established for the simultaneous determination of seven chlorogenic acids in plant beverages,by using C18 chromatographic column,elutioned with acetonitrile+0.1%formic acid+0.1%acetic acid.The detection wavelength was 327 nm and the reference wavelength was 400 nm.The detection limit of seven chlorogenic acids was 0.25 mg/L,and the relationship was linear in the concentration range of 0.5~20µg/mL.The recoveries of neochloro genic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,1,5-dicaveoyl quinic acid,isochlorogenic acid A and isochlorogenic acid C were 99%~108%,97.9%~101%,96.9%~102%,96.2%~106%,95.1%~96.7%,94.9%~99.6%,96.3%~108%,respectively.The method was simple,fast,accurate in qualitative and quantitative,and could simultaneously determine seven kinds of chlorogenic acids in plant beverages.

关 键 词：高效液相色谱法 植物饮料 7种绿原酸 

分 类 号：TQ463[化学工程—制药化工]