Hemodialysis bilayer bionic blood vessels developed by the mechanical stimulation of hepatitis B viral X(HBX)genetransfected hepatic stellate cells  

作　　者：Hongyi LIU Yuanyuan ZHOU Peng GUO Xiongwei ZHENG Weibin CHEN Shichao ZHANG Yu FU Xu ZHOU Zheng WAN Bin ZHAO Yilin ZHAO 

机构地区：[1]Department of Oncology and Vascular Interventional Radiology,Zhongshan Hospital Affiliated to Xiamen University,School of Medicine,Xiamen University,Xiamen 361004,China [2]School of Medicine,Xiamen University,Xiamen 361102,China [3]Xiamen Health and Medical Big Data Center,Xiamen 361008,China [4]Fujian Provincial Key Laboratory of Chronic Liver Disease and Hepatocellular Carcinoma(Zhongshan Hospital Affiliated to Xiamen University),Xiamen 361004,China [5]Xiamen Key Laboratory of Cellular Intervention and Interventional Medical Materials,Xiamen 361004,China

出　　处：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》2024年第6期499-512,共14页浙江大学学报（英文版）B辑（生物医学与生物技术）

基　　金：supported by the National Natural Science Foundation of China(No.81770294);the Natural Science Foundation of Fujian Province(No.2023J05261),China.

摘　　要：Artificial vascular graft(AVG)fistula is widely used for hemodialysis treatment in patients with renal failure.However,it has poor elasticity and compliance,leading to stenosis and thrombosis.The ideal artificial blood vessel for dialysis should replicate the structure and components of a real artery,which is primarily maintained by collagen in the extracellular matrix(ECM)of arterial cells.Studies have revealed that in hepatitis B virus(HBV)-induced liver fibrosis,hepatic stellate cells(HSCs)become hyperactive and produce excessive ECM fibers.Furthermore,mechanical stimulation can encourage ECM secretion and remodeling of a fiber structure.Based on the above factors,we transfected HSCs with the hepatitis B viral X(HBX)gene for simulating the process of HBV infection.Subsequently,these HBX-HSCs were implanted into a polycaprolactonepolyurethane(PCL-PU)bilayer scaffold in which the inner layer is dense and the outer layer consists of pores,which was mechanically stimulated to promote the secretion of collagen nanofiber from the HBX-HSCs and to facilitate crosslinking with the scaffold.We obtained an ECM-PCL-PU composite bionic blood vessel that could act as access for dialysis after decellularization.Then,the vessel scaffold was implanted into a rabbit’s neck arteriovenous fistula model.It exhibited strong tensile strength and smooth blood flow and formed autologous blood vessels in the rabbit’s body.Our study demonstrates the use of human cells to create biomimetic dialysis blood vessels,providing a novel approach for creating clinical vascular access for dialysis.

关 键 词：Composite bilayer bionic blood vessel Extracellular matrix(ECM) Hepatic stellate cells(HSCs) Hepatitis B viral X(HBX)gene Mechanical force 

分 类 号：R730.3[医药卫生—肿瘤]