白术内酯Ⅲ减轻脑梗死模型大鼠神经功能损伤  

Atractylodes macrocephala lactoneⅢalleviates nerve function injury in rat model with cerebral infarction

作　　者：张秋萍 杨月君[2] 张彩丽[3] ZHANG Qiuping;YANG Yuejun;ZHANG Caili(Department of Outpatient,the NO.2 Hospital of Baoding,Baoding 071051,China;Department of Neurology,the NO.2 Hospital of Baoding,Baoding 071051,China;Department of Obstetrics and Gynaecology,the NO.2 Hospital of Baoding,Baoding 071051,China)

机构地区：[1]保定市第二医院门诊部,河北保定071051 [2]保定市第二医院神经内科,河北保定071051 [3]保定市第二医院妇产科,河北保定071051

出　　处：《基础医学与临床》2024年第8期1143-1148,共6页Basic and Clinical Medicine

基　　金：保定市科技计划项目(17ZF152)。

摘　　要：目的探究白术内酯Ⅲ(AMLⅢ)对脑梗死大鼠神经功能损伤的影响。方法将大鼠随机分为假手术组(sham)、模型组(model,改良Longa线栓法制备大鼠脑梗死模型)、白术内酯Ⅲ低和高剂量组(AMLⅢ-L、AMLⅢ-H)、白术内酯Ⅲ高剂量+EX527(SIRT1抑制剂)组(AMLⅢ-H-EX527)。Zea-Longa评分法评估神经功能,ELISA检测血清肿瘤坏死因子(TNF-α)、白细胞介素(IL-6、IL-1β)水平;试剂盒检测脑组织超氧化物歧化酶(SOD)活性、丙二醛(MDA)含量、过氧化氢酶(CAT)活性;HE染色观察海马组织病理,TTC染色法检测脑梗死面积,TUNEL染色法检测脑细胞凋亡率;Western blot检测脑组织B淋巴细胞瘤(Bcl-2)、Bcl-2关联X蛋白(Bax)、SIRT1、Nrf2蛋白表达。结果白术内酯Ⅲ干预后可减轻大鼠海马神经组织损伤,降低神经功能评分、血清TNF-α、IL-6和IL-1β水平、凋亡率、脑梗死面积、MDA水平、Bax蛋白表达,提高SOD和CAT活性、SIRT1、Nrf2、Bcl-2蛋白表达水平(P<0.05);EX527可减轻白术内酯Ⅲ对脑梗死大鼠神经功能的保护作用。结论白术内酯Ⅲ可减轻脑梗死模型大鼠的神经功能损伤。Objective To explore the effect of atractylodes macrocephala lactoneⅢ(AMLⅢ)on nerve injury of rats with cerebral infarction.Methods The rats were randomly divided into sham operation group,model group(modified Longa thrombus method to prepare rat cerebral infarction model),low and high dose of AMLⅢgroup(AMLⅢ-L,AMLⅢ-H)and high dose AMLⅢ+EX527(SIRT1 inhibitor)group(AMLⅢ-H-EX527).Neurological function was assessed by Zea-Longa score.Serum tumor necrosis factor(TNF-α)and interleukin(IL-6,IL-1β)were measured by ELISA;The level of super oxide dismutase activity(SOD),malonaldehyde content(MDA),and catalase activity(CAT)in brain tissue was detected by commercially available kit;pathological changes of hippocampus tissue and the area of cerebral infarction were observed by HE staining and TTC staining microscopy respectively.Apoptosis of brain cells was identified by TUNEL staining;protein expression of Bax,Bcl-2,SIRT1,and Nrf2 in rat brain tissue was detected by Western blot assay.Results After the intervention by AMLⅢ,the pathological injury of hippocampus neurons in rats was alleviated.The neurological function score,serum TNF-α,IL-6 and IL-1βlevels,apoptosis rate,cerebral infarction size,MDA level and Bax protein expression were all decreased.SOD and CAT activity,SIRT1,Nrf2,Bcl-2 and protein expression were all increased(P<0.05);EX527 was able to alleviate the protective effect of AMLⅢon neural function damage in rats with cerebral infarction.Conclusions Atractylodes macrocephala lactoneⅢreduces the nerve function injury in rat models with cerebral infarction.

关 键 词：白术内酯Ⅲ 脑梗死 神经功能损伤 Zea-Longa评分 

分 类 号：R743[医药卫生—神经病学与精神病学]