Box-Behnken响应面法优化复合益生菌发酵三七药渣工艺及体外抗氧化活性研究  

Optimization of Composite Probiotics Fermentation Process for Notoginseng Radix et Rhizoma Residues by Box-Behnken Response Surface Method and Evaluation of Their in vitro Antioxidant Activities

作　　者：王昱[1] 郭盛[1] 刘海峰 钱大玮[1] 王明耿 郭兰萍[3] 段金廒[1] WANG Yu;GUO Sheng;LIU Haifeng;QIAN Dawei;WANG Minggeng;GUO Lanping;DUAN Jinao(National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,National Administration of Traditional Chinese Medicine Key Laboratory for Chinese Medicine Resources Recycling Utilization,Nanjing University of Chinese Medicine,Nanjing 210023,China;Shandong Buchang Pharmaceutical Co.,Ltd,Heze 274000,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-Di Herbs,Beijing 100700,China)

机构地区：[1]南京中医药大学中药资源产业化与方剂创新药物国家地方联合工程研究中心/江苏省中药资源产业化过程协同创新中心/国家中医药管理局中药资源循环利用重点研究室,江苏南京210023 [2]山东步长制药股份有限公司,山东菏泽274000 [3]中国中医科学院道地药材品质保障与资源持续利用全国重点实验室,北京100700

出　　处：《南京中医药大学学报》2024年第6期555-568,共14页Journal of Nanjing University of Traditional Chinese Medicine

基　　金：山东省重点研发计划项目(2021SFGC1203);国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202005);中央本级重大增减支项目(2060302);中医药传承与创新“百千万”人才工程(岐黄工程)岐黄学者项目(2019)。

摘　　要：目的以三七药渣为原料,通过益生菌发酵比较不同益生菌以及复合益生菌对各指标成分影响的差异,探究最佳发酵工艺。方法采用单因素、Box-Behnken响应面法优化发酵工艺,并通过体外抗氧化实验评价发酵产物的抗氧化能力。结果最佳发酵工艺为有氧发酵48 h、厌氧发酵36 h,嗜热链球菌、两歧双歧杆菌、嗜酸乳杆菌的比例为2∶3∶1,料液比0.14 g·mL^(-1),接菌量5%,温度33℃。在最佳发酵工艺条件下,三七药渣中性多糖、酸性多糖、总黄酮的含量分别提高了105.64%、96.98%、123.83%,相较于单菌发酵均显著升高。发酵产物清除DPPH、ABTS自由基的IC 50值分别为1.774、3.065 mg·mL^(-1),还原Fe^(3+)的能力为0.138 mmol FeSO_(4)·g^(-1),较未发酵药渣显著增强。结论最佳发酵工艺能显著提高三七药渣中各指标成分含量,显著增强其抗氧化能力,相较单菌发酵各指标成分含量均显著提高,提示上述益生菌之间没有明显的拮抗作用。研究结果为三七药渣的资源化利用提供了科学依据和数据支撑。OBJECTIVE Notoginseng Radix et Rhizoma residues were used as raw material to compare the difference in the impact of different probiotics and composite probiotic on various indicator components through probiotic fermentation,aiming to explore the optimal fermentation process.METHODS The fermentation process was optimized using single factor and Box-Behnken response surface methodology,and the antioxidant capacity of the fermentation product was assessed through in vitro antioxidant experiments.RESULTS The results showed the optimum fermentation processes were 48 h of aerobic fermentation,36 h of anaerobic fermentation,ratio of 2∶3∶1 for Streptococcus thermophilus,Bifidobacterium bifidum and Lactobacillus acidophilus,solid-liquid ratio of 0.14 g·mL^(-1),inoculation quantity 5%,fermentation temperature 33℃.Under the optimal fermentation conditions,the content of neutral polysaccharide,acidic polysaccharide,and total flavonoid in Notoginseng Radix et Rhizoma residue increased by 105.64%,96.98% and 123.83%,respectively,which were high than those single-strain fermentation.The IC 50 values of scavenging DPPH and ABTS free radicals in the fermentation products were 1.774 mg·mL^(-1) and 3.065 mg·mL^(-1),respectively,and the power of reducing Fe^(3+)was 0.138 mmol FeSO_(4) g^(-1).The antioxidant capacity was significantly enhanced compared to the unfermented residues.CONCLUSION The optimum fermentation process can significantly elevate the content of indicator components in Notoginseng Radix et Rhizoma residues and enhance its antioxidant capacity.Compared to single-strain fermentation,the content of various indicator components is significantly increased,showing no apparent antagonistic effect among the probiotics mentioned above.The study provides scientific evidence and data support for the resource utilization of Notoginseng Radix et Rhizoma residues.

关 键 词：三七药渣 益生菌 层次分析法 熵权法 体外抗氧化 

分 类 号：R282.4[医药卫生—中药学]