Tumor targeted and GSH stimuli response a-lactalbumin nanotubes co-delivering doxorubicin and siRNA for cancer therapy  

作　　者：Guohua Hou Di Wu Xing Li Bin Liu 

机构地区：[1]Research Center of Food Colloids and Delivery of Functionaliny,Collge of Food Science and Mutritional Engineering,China Agriculnrral Universin,Beijimg 10083,China [2]Department of Numition cand Heaith,China Agricultural University,Beijing 100193,China [3]Deparoment of Health Sciences and Technology.Eidgenossische Technische Hochschule Zinich,Zurich 8092,Switzerland

出　　处：《Journal of Future Foods》2024年第4期300-308,共9页未来食品学报（英文）

基　　金：supported by the China National Postdoctoral Program for Innovative Talents (BX2021364);the China Postdoctoral Science Foundation (2021M703525).

摘　　要：α-Lactalbumin(a-lac),a natural food-sourced protein,exhibits high biocompatibility as delivery systems for small bioactive molecules or RNA.However,their potential clinical application as a delivery system is greatly limited due to the 1ack of targeting to desired tissues,such as tumors.Here,a delivery system/nanocarier of nantube(NT)was fabricated by the self-assembly of peptides hydrolyzed from a-1ac.Among the 1oading of anti-tumor agents of doxorubicin(Dox)and siRNA,NTs were conjugated with the tLyp-1 peptide to specifially target the neuropilin 1 receptor,which exists on the membrane of MDA-MB-231 cancer cells.The micromonphology of NTs was not affected during the processes of 1oading and targeting modification.Additionally,no significant changes in the size and zeta potential of the tI yp-1/NTs/Dox/siRNA nanocarrier were observed when stored in phosphate buffered saline for 7 days,indicating outstanding stability.Moreover,both the cellular uptake efficiency and tumor accumulation effects of NTs on MDA-MB-231 breast cancer cells were significantly improved after modification with tLyp-1.The relative viability of MDA-MB-231 cells was decreased by 70.1%in viro after treatmnent with tLyp-1/NTs/Dox/siRNA(5μg/mL Dox).Finally,a synergistic anticancer effect of Dox and siRNA co-loaded in tLyp-1/NTs was achieved in the anticancer treatment of MDA-MB-231 tumor-bearing mice.These findings indicate that food-sourced protein a-lac nanocarriers could be used for the co-delivery of bioactive molecules and RNA,achieving synergistic and effcient therapeutic effects.

关 键 词：α-Lactabumin nanotube Nanocarier Tumor-targeted SIRNA Synergistic effect 

分 类 号：R730.5[医药卫生—肿瘤]