猪繁殖与呼吸综合征病毒作为外源基因表达活载体的研究进展  

Research advance in porcine reproductive and respiratory syndrome virus as a live vector to express foreign genes

作　　者：王树茂 王桂花[1] 王安冉 段宏勇 姜一峰[2] 周艳君[2] 高飞[2,3] 李丽薇 WANG Shumao;WANG Guihua;WANG Anran;DUAN Hongyong;JIANG Yifeng;ZHOU Yanjun;GAO Fei;LI Liwei(College of Veterinary Medicine,Shandong Agricultural University,Tai'an 271018,China;Shanghai Veterinary Research Institute,Chinese Academy of Agricultural Sciences,Shanghai 200241,China;Jiangsu Co-innovation Center for the Prevention and Control of Important Animal Infectious Disease and Zoonoses,Yangzhou 225009,China)

机构地区：[1]山东农业大学动物科技学院,山东泰安271018 [2]中国农业科学院、上海兽医研究所,上海200241 [3]江苏省动物重要疫病与人兽共患病防控协同创新中心,江苏扬州225009

出　　处：《中国兽医科学》2024年第6期816-823,共8页Chinese Veterinary Science

基　　金：国家重点研发计划项目(2022YFD1800305,2022YFD1800804,2021YFD1801401);上海市科技兴农项目(2022-02-08-00-12-F01162);中国农业科学院科技创新工程项目(CAAS-CSLPDCP-202301)。

摘　　要：自美国(1987年)和欧洲(1990年)首次分离猪繁殖与呼吸综合征病毒(PRRSV)以来,该病毒在全球流行已超过30年。直至今日,PRRSV仍然不断变异和进化,在我国的流行态势尤其复杂,在后非洲猪瘟时代仍然是影响养猪业的最具经济意义的病原体之一。目前,市售疫苗包括传统灭活病毒疫苗(KV)和改良活病毒疫苗(MLV)。然而,PRRSV的疫苗预防和控制尚未完全实现。当前迫切需要开发一种安全、有效、通用的基因工程疫苗。基于PRRSV感染性克隆反向遗传平台表达外源基因,包括标记基因、免疫增强基因或其他病毒抗原基因,构建重组PRRSV疫苗、基因缺失疫苗、广谱通用疫苗等成为了研究的热点。在此,我们综述了近年来PRRSV作为病毒活载体表达外源基因的研究进展及其在新型基因工程疫苗开发中的潜在应用前景。Since the first isolation of porcine reproductive and respiratory syndrome virus(PRRSV)in the United States(1987)and Europe(1990),the virus has been circulating globally for over 30years.Until today,PRRSV continues to mutate and evolve,and its epidemic situation in China is particularly complex.In the post African swine fever era,it remains one of the most economically significant pathogens affecting the pig farming industry.Currently,commercially available vaccines include traditional inactivated virus vaccines(KV)and modified live virus vaccines(MLV).However,vaccine prevention and control of PRRSV have not yet been fully implemented.There is an urgent need to develop a safe,effective,and universal genetic engineering vaccine.Based on PRRSV infectious cloning reverse genetic platform,the expression of foreign genes,including marker genes,immune enhancing genes,or other viral antigen genes,has become a research hotspot.The construction of recombinant vaccines,gene deletion vaccines,and broad-spectrum universal vaccines is needed.Herein,we review the research advance of PRRSV as a live vector for expressing foreign genes and its potential application prospects in the development of new genetic engineering vaccines in recent years.

关 键 词：猪繁殖与呼吸综合征病毒(PRRSV) 基因工程疫苗 感染性克隆 外源基因 

分 类 号：S852.659.6[农业科学—基础兽医学]