HPLC-DAD法同时测定褐变金银花中15种活性成分含量  

The HPLC-DAD method simultaneously determined the content of 15 active ingredients in browned Honeysuckle

作　　者：孙丹 于晓[2] 段言峰 范剑[2] 张龙霏 张芳[1] 张永清[1] 李佳[1] 刘玉红[1] SUN Dan;YU Xiao;DUAN Yan-feng;FAN Jian;ZHANG Long-fei;ZHANG Fang;ZHANG Yong-qing;LI Jia;LIU Yu-hong(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Ji'nan 250355,China;Shandong Medicine Technician College,Taian 271016,China;Puruite Machinery Manufacturing Co.,Ltd,Taian 271016,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]山东医药技师学院,山东泰安271016 [3]普瑞特机械制造股份有限公司,山东泰安271016

出　　处：《时珍国医国药》2024年第5期1101-1105,共5页Lishizhen Medicine and Materia Medica Research

基　　金：山东省中医药科技项目(2020Z02);山东省泰安市科技创新重大专项(2022ZDZX026);山东省自然科学基金项目(ZR2021QH238);山东省重点研发计划项目(2022TZXD0036);泰山产业领军人才工程专项(tscy20200410)。

摘　　要：目的阐释褐变对金银花药材质量的影响。方法采用HPLC-DAD法比较褐变和正常金银花样品中15种成分(5种酚酸类成分、4种黄酮类成分、6种环烯醚萜苷类成分)的含量,采用SIMCA14.1软件进行主成分(PCA)、正交偏最小二乘法分析(OPLS-DA)。结果褐变后,金银花中酚酸类成分、黄酮类成分及15种成分的总含量均显著下降,环烯醚萜苷类成分中断氧化马钱子苷、莫诺苷和獐牙菜苷含量及6种环烯醚萜苷类成分总含量也显著下降。PCA和OPLS-DA分析结果表明,正常样品与褐变样品各聚为一类,差异显著,VIP分析结果表明,绿原酸、异绿原酸A、断马钱子酸是区别正常样品和褐变样品的主要差异成分。结论褐变会导致金银花药材质量下降,在产地加工过程中应采用适宜方法降低褐变发生率,提升临床用金银花质量。Objective Browning is an important factor affecting the quality of Honeysuckle,which lays a foundation for comprehensively explaining the influence of browning on the quality of Honeysuckle.Methods In this study,15 components including 5 phenolic acid components,4 flavonoids and 6 iridoid glycosides were compared using HPLC-DAD methods.Results The results showed that the total content of 15 components,5 phenolic acids,4 flavonoids and 6 iridoid glycosides decreased significantly in Browning Lonicerae japonicae Flos.The results of PCA and OPLS-DA analysis showed that the normal and Browning samples differed significantly,and were clustered into different groups respectively,and the analysis of VIP value of the OPLS-DA model showed that chlorogenic acid,isochlorogenic acid A and secologanic acid were the main differential components between the normal and Browning samples.Conclusion Browning will reduce the quality of Honeysuckle.Appropriate methods should be adopted to reduce the browning rate and improve the quality of Honeysuckle for clinical use in the processing of producing areas.

关 键 词：金银花 褐变 环烯醚萜苷 酚酸 黄酮 

分 类 号：R284.1[医药卫生—中药学]