响应面法对金银花叶总黄酮与绿原酸同步萃取工艺的优化  

Optimization of the Simultaneous Extraction Process of Total Flavonoids andChlorogenic Acid from Honeysuckle Leaves by Response Surface Method

作　　者：李华生[1] 周振华[1] 骆航[1] 龙鸣[1] 陈瑛[1] Li Huasheng;Zhou Zhenhua;Luo Hang;Long Ming;Chen Ying(Yongzhou Vocational and Technical College,Yongzhou 425100,China)

机构地区：[1]永州职业技术学院,湖南永州425100

出　　处：《山东化工》2024年第12期4-8,12,共6页Shandong Chemical Industry

基　　金：湖南省教育厅2022年科研项目(22C1344)。

摘　　要：采用响应面法,对加压辅助溶剂同步萃取金银花叶总黄酮和绿原酸的工艺进行了优化。在单因素试验的基础上,综合评价总黄酮和绿原酸的得率“OD值”作为因变量,通过响应面法优化了液料比、萃取压力、溶剂体积分数、萃取温度和萃取时间。结果表明,优化后的加压辅助溶剂同步萃取工艺参数为:液料比26.5 mL/g,萃取压力3.1 MPa,溶剂体积分数60.5%,萃取温度90.3℃,萃取时间32 min。该工艺条件下,从金银花叶中萃取总黄酮得率为205.3 mg/g,绿原酸得率为24.5 mg/g, OD值与理论预测值的相对误差为1.0%。The process of simultaneous extraction of total flavonoids and chlorogenic acid from honeysuckle leaves by pressurized auxiliary solvent was optimized by using the response surface method.On the basis of single factor experiment,the yield of total flavonoids and chlorogenic acid was comprehensively evaluated as the dependent variable,and the liquid-material ratio,extraction pressure,solvent volume fraction,extraction temperature and extraction time were optimized by response surface method.The results showed that the optimized process parameters of pressurized auxiliary solvent synchronous extraction were as follows:liquid-material ratio 26.5 mL/g,extraction pressure 3.1 MPa,solvent volume fraction 60.5%,extraction temperature 90.3℃,and extraction time 32 min.Under the conditions of this process,the yield of total flavonoids extracted from honeysuckle leaves was 205.3 mg/g,the yield of chlorogenic acid was 24.5 mg/g,and the relative error between OD value and theoretical predicted value was 1.0%.

关 键 词：响应面分析 金银花叶 总黄酮 绿原酸 加压萃取工艺 

分 类 号：TQ461[化学工程—制药化工] TS05[轻工技术与工程] TS202.3